LOCUS BBa_S01999 5483 bp DNA linear 23-Dec-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01999 VERSION BBa_S01999.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 CDS 127..743 /label=tetR misc_feature 744..777 /label=SsrA misc_feature 784..808 /label=Help:Barcodes CDS 124..783 /label=BBa_C0040 stem_loop 828..871 /label=stem_loop misc_feature 817..896 /label=BBa_B0010 terminator 817..896 /label=BBa_B0010 stem_loop 912..931 /label=T7 TE misc_feature 938..938 /label=stop polyA_site 932..945 /label=polya misc_feature 905..945 /label=BBa_B0012 terminator 905..945 /label=BBa_B0012 misc_feature 954..972 /label=TetR 1 misc_feature 954..1007 /label=BBa_R0040 promoter 973..978 /label=-35 misc_feature 979..997 /label=TetR 2 promoter 996..1001 /label=-10 promoter 954..1007 /label=BBa_R0040 misc_feature 1020..1023 /label=conserved RBS 1016..1027 /label=BBa_B0034 misc_feature 2123..2161 /label=LVA CDS 1034..2161 /label=lacI-LVA misc_feature 1034..2161 /label=BBa_C0012 misc_feature 2162..2186 /label=Help:Barcodes CDS 1034..2161 /label=BBa_C0012 stem_loop 2206..2249 /label=stem_loop misc_feature 2195..2274 /label=BBa_B0010 terminator 2195..2274 /label=BBa_B0010 stem_loop 2290..2309 /label=T7 TE misc_feature 2316..2316 /label=stop polyA_site 2310..2323 /label=polya misc_feature 2283..2323 /label=BBa_B0012 terminator 2283..2323 /label=BBa_B0012 CDS 2332..2419 /label=end of LacI coding region (inactive) misc_feature 2332..2531 /label=BBa_R0010 misc_feature 2420..2457 /label=CAP binding site promoter 2468..2473 /label=-35 promoter 2492..2497 /label=-10 misc_feature 2497..2531 /label=LacI binding site misc_feature 2504..2504 /label=start promoter 2332..2531 /label=BBa_R0010 misc_feature 2544..2547 /label=conserved RBS 2540..2551 /label=BBa_B0034 CDS 2561..3268 /label=cI lambda misc_feature 3269..3301 /label=LVA misc_feature 3308..3332 /label=Help:Barcodes CDS 2558..3307 /label=BBa_C0051 stem_loop 3352..3395 /label=stem_loop misc_feature 3341..3420 /label=BBa_B0010 terminator 3341..3420 /label=BBa_B0010 stem_loop 3436..3455 /label=T7 TE misc_feature 3462..3462 /label=stop polyA_site 3456..3469 /label=polya misc_feature 3429..3469 /label=BBa_B0012 terminator 3429..3469 /label=BBa_B0012 misc_feature 3478..3574 /label=BBa_R0065 misc_feature 3483..3502 /label=Lux Box promoter 3524..3529 /label=-10 misc_feature 3534..3550 /label=OR2 cI promoter 3535..3535 /label=lux p(R) start promoter 3548..3553 /label=-35 misc_feature 3558..3574 /label=OR1 cI promoter 3571..3574 /label=-10 promoter 3478..3574 /label=BBa_R0065 misc_feature 3587..3590 /label=conserved RBS 3583..3594 /label=BBa_B0034 misc_feature 4180..4211 /label=LVA CDS 3601..4179 /label=luxI misc_feature 3601..4218 /label=BBa_C0061 misc_feature 4219..4243 /label=Help:Barcodes CDS 3601..4218 /label=BBa_C0061 stem_loop 4263..4306 /label=stem_loop misc_feature 4252..4331 /label=BBa_B0010 terminator 4252..4331 /label=BBa_B0010 stem_loop 4347..4366 /label=T7 TE misc_feature 4373..4373 /label=stop polyA_site 4367..4380 /label=polya misc_feature 4340..4380 /label=BBa_B0012 terminator 4340..4380 /label=BBa_B0012 misc_feature 4389..4408 /label=LuxR/HSL promoter 4503..4510 /label=-10 promoter 4477..4482 /label=-35 misc_feature 4516..4516 /label=start misc_feature 4518..4537 /label=Putative LuxR/HSL misc_feature 4389..4539 /label=BBa_R0063 promoter 4389..4539 /label=BBa_R0063 misc_feature 4552..4555 /label=conserved RBS 4548..4559 /label=BBa_B0034 misc_feature 4566..4567 /label=prefix misc_feature 4739..4739 /label=T misc_feature 5057..5057 /label=A CDS 4566..5315 /label=luxR misc_feature 4566..5321 /label=BBa_C0062 misc_feature 5322..5346 /label=Help:Barcodes CDS 4566..5321 /label=BBa_C0062 stem_loop 5366..5409 /label=stem_loop misc_feature 5355..5434 /label=BBa_B0010 terminator 5355..5434 /label=BBa_B0010 stem_loop 5450..5469 /label=T7 TE misc_feature 5476..5476 /label=stop polyA_site 5470..5483 /label=polya misc_feature 5443..5483 /label=BBa_B0012 terminator 5443..5483 /label=BBa_B0012 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag 181 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct 241 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg 301 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta 361 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta 421 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt 481 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat 541 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa 601 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac 661 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa 721 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa 781 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat 961 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga 1021 ggagaaatac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 1081 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 1141 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 1201 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 1261 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 1321 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 1381 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 1441 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 1501 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 1561 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 1621 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 1681 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 1741 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 1801 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 1861 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 1921 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 1981 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 2041 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 2101 ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata 2161 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct 2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2281 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gcaatacgca 2341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 2401 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 2461 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 2521 aatttcacac atactagaga aagaggagaa atactagatg agcacaaaaa agaaaccatt 2581 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa 2641 tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt 2701 tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa 2761 aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat 2821 gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc 2881 tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga 2941 gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg 3001 taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct 3061 cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga 3121 tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa 3181 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc 3241 tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg ctttagtagc 3301 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 3361 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 3421 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtaa 3481 gcacctgtag gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataacacc 3541 gtgcgtgttg actattttac ctctggcggt gatatactag agaaagagga gaaatactag 3601 atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga gtataaaggt 3661 attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt agttgtagaa 3721 aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta tgcttgtgat 3781 gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga ttatatgctg 3841 aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc taatatagtc 3901 gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc tgctagtgaa 3961 attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg tattacagaa 4021 tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa agttccttgt 4081 catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt tgtattgtct 4141 atgcctatta atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga cgaaaactac 4201 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc 4261 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 4321 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 4381 tactagagac ctgtacgatc ctacaggtgc ttatgttaag taattgtatt cccagcgata 4441 caatagtgtg acaaaaatcc aatttattag aatcaaatgt caatccatta ccgttttaat 4501 gatatataac acgcaaaact tgcgacaaac aataggtaat actagagaaa gaggagaaat 4561 actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt 4621 gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg 4681 aatattattt actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc 4741 tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg 4801 atcctatagt agattattct aactccaatc attcaccaat taattggaat atatttgaaa 4861 acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca tcaggtctta 4921 tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg cttagttttg 4981 cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt atgaacatac 5041 cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca aataataaat 5101 caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa 5161 gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact ttccatttaa 5221 ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct aaagcaattt 5281 taacaggagc aattgattgc ccatacttta aaaattaata acactgatag tgctagtgta 5341 gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 5401 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 5461 gggtgggcct ttctgcgttt ata //