LOCUS BBa_S03165 2065 bp DNA linear 22-Nov-2024 DEFINITION R0011.B0034.C0051.B0034.C0061.E0433 ACCESSION BBa_S03165 VERSION BBa_S03165.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..830 /label=BBa_C0051 misc_feature 868..871 /label=conserved RBS 864..875 /label=BBa_B0034 misc_feature 1461..1492 /label=LVA CDS 882..1460 /label=luxI misc_feature 882..1499 /label=BBa_C0061 misc_feature 1500..1524 /label=Help:Barcodes CDS 882..1499 /label=BBa_C0061 misc_feature 1537..1540 /label=conserved RBS 1533..1544 /label=BBa_B0034 CDS 1551..1898 /label=lacZ alpha misc_feature 1631..1631 /label=T misc_feature 1637..1637 /label=A misc_feature 1658..1658 /label=C misc_feature 1628..1628 /label=C misc_feature 1661..1661 /label=G promoter 1576..1585 /label=T3 promoter promoter 1723..1741 /label=T7 promoter misc_feature 1749..1898 /label=lacZ gene fragment misc_feature 1551..1565 /label=lacZ gene fragment misc_feature 1551..1903 /label=BBa_E0033 stem_loop 1948..1991 /label=stem_loop misc_feature 1937..2016 /label=BBa_B0010 terminator 1937..2016 /label=BBa_B0010 stem_loop 2032..2051 /label=T7 TE misc_feature 2058..2058 /label=stop polyA_site 2052..2065 /label=polya misc_feature 2025..2065 /label=BBa_B0012 terminator 2025..2065 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagaaagagg agaaatacta gatgactata atgataaaaa 901 aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc 961 aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg 1021 agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg 1081 gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat 1141 tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg 1201 taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg 1261 aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa 1321 cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag 1381 aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt 1441 ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac 1501 tctgatagtg ctagtgtaga tctctactag agaaagagga gaaatactag atgaccatga 1561 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg 1621 tggcggcagc actagagcta gtggatcccc cgggctgtag aaattcgata tcaagcttat 1681 cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt 1741 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 1801 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 1861 gcaccgatcg cccttcccaa cagttgcgca gcctgaataa taacgctgat agtgctagtg 1921 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 1981 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2041 tcgggtgggc ctttctgcgt ttata //