LOCUS BBa_S03167 2176 bp DNA linear 25-Nov-2024 DEFINITION Q04121.I0461 ACCESSION BBa_S03167 VERSION BBa_S03167.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 stem_loop 1191..1234 /label=stem_loop misc_feature 1180..1259 /label=BBa_B0010 terminator 1180..1259 /label=BBa_B0010 stem_loop 1275..1294 /label=T7 TE misc_feature 1301..1301 /label=stop polyA_site 1295..1308 /label=polya misc_feature 1268..1308 /label=BBa_B0012 terminator 1268..1308 /label=BBa_B0012 misc_feature 1319..1335 /label=lac O1 promoter 1336..1341 /label=-35 misc_feature 1342..1358 /label=lac O1 promoter 1359..1364 /label=-10 misc_feature 1317..1370 /label=BBa_R0011 promoter 1317..1370 /label=BBa_R0011 misc_feature 1383..1386 /label=conserved RBS 1379..1390 /label=BBa_B0034 misc_feature 1976..2007 /label=LVA CDS 1397..1975 /label=luxI misc_feature 1397..2014 /label=BBa_C0061 misc_feature 2015..2039 /label=Help:Barcodes CDS 1397..2014 /label=BBa_C0061 stem_loop 2059..2102 /label=stem_loop misc_feature 2048..2127 /label=BBa_B0010 terminator 2048..2127 /label=BBa_B0010 stem_loop 2143..2162 /label=T7 TE misc_feature 2169..2169 /label=stop polyA_site 2163..2176 /label=polya misc_feature 2136..2176 /label=BBa_B0012 terminator 2136..2176 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac tactagagaa 1381 agaggagaaa tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc 1441 ggaggagtat aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg 1501 ggacttagtt gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata 1561 tatttatgct tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac 1621 aggtgattat atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa 1681 agatcctaat atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa 1741 taactctgct agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag 1801 tcaaggtatt acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg 1861 tattaaagtt ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa 1921 atcggttgta ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaatgctgc 1981 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct 2041 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 2101 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 2161 cctttctgcg tttata //