##gff-version 3
##sequence-region BBa_S03174 1 1031
BBa_S03174	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03174	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03174	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S03174	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S03174	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S03174	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S03174	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S03174	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S03174	.	terminator	991	1031	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03174	.	engineered_region	991	1031	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03174	.	stop_codon	1024	1024	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03174	.	polyA_site	1018	1031	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03174	.	stem_loop	998	1017	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03174	.	CDS	81	869	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_S03174	.	stop_codon	864	869	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S03174	.	start_codon	81	83	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S03174	.	CDS	81	830	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S03174	.	engineered_region	81	869	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S03174	.	tag	831	863	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S03174	.	engineered_tag	870	894	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S03174	.	terminator	903	982	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03174	.	stem_loop	914	957	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03174	.	engineered_region	903	982	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S03174
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcactactagagaaagaggagaaatacta
gatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaaca
ccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggta
tgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactga
agaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttg
cattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgc
agcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgt
accaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggt
cctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcag
aattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatat
agagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataa
cgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata