LOCUS BBa_S03175 3067 bp DNA linear 09-May-2024 DEFINITION Subassembly for CalTech I12028 (S11451.S50452) ACCESSION BBa_S03175 VERSION BBa_S03175.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..830 /label=BBa_C0051 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 promoter 2080..2210 /label=Pbad promoter 2043..2114 /label=PC misc_feature 1001..2079 /label=AraC promoter 1001..2210 /label=BBa_I0500 misc_feature 2223..2226 /label=conserved RBS 2219..2230 /label=BBa_B0034 CDS 2237..2905 /label=cI 434 misc_feature 2867..2905 /label=LVA misc_feature 2237..2905 /label=BBa_C0052 misc_feature 2906..2930 /label=Help:Barcodes CDS 2237..2905 /label=BBa_C0052 stem_loop 2950..2993 /label=stem_loop misc_feature 2939..3018 /label=BBa_B0010 terminator 2939..3018 /label=BBa_B0010 stem_loop 3034..3053 /label=T7 TE misc_feature 3060..3060 /label=stop polyA_site 3054..3067 /label=polya misc_feature 3027..3067 /label=BBa_B0012 terminator 3027..3067 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttatgacaac ttgacggcta 1021 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt 1081 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg 1141 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc 1201 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg 1261 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc 1321 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa 1381 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat 1441 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct 1501 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc 1561 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct 1621 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc 1681 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga 1741 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct 1801 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat 1861 cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc 1921 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac 1981 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa 2041 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg 2101 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct 2161 ttttatcgca actctctact gtttctccat acccgttttt ttgggctagc tactagagaa 2221 agaggagaaa tactagatga gtatttcttc cagggtaaaa agcaaaagaa tccagcttgg 2281 acttaaccag gctgaacttg ctcaaaaggt ggggactacc cagcagtcta tagagcagct 2341 cgaaaacggt aaaactaagc gaccacgctt tttaccagaa cttgcgtcag ctcttggcgt 2401 aagtgttgac tggctgctca atggcacctc tgattcgaat gttagatttg ttgggcacgt 2461 tgagcccaaa gggaaatatc cattgattag catggttaga gctggttcgt ggtgtgaagc 2521 ttgtgaaccc tacgatatca aggacattga tgaatggtat gacagtgacg ttaacttatt 2581 aggcaatgga ttctggctga aggttgaagg tgattccatg acctcacctg taggtcaaag 2641 catccctgaa ggtcatatgg tgttagtaga tactggacgg gagccagtga atggaagcct 2701 tgttgtagcc aaactgactg acgcgaacga agcaacattc aagaaactgg tcatagatgg 2761 cggtcagaag tacctgaaag gcctgaatcc ttcatggcct atgactccta tcaacggaaa 2821 ctgcaagatt atcggtgttg tcgtggaagc gagggtaaaa ttcgtagctg caaacgacga 2881 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc 2941 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3001 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3061 gtttata //