LOCUS       BBa_S03176              1888 bp    DNA     linear       22-Dec-2024
DEFINITION  R0011.E0420.R0040.E0430
ACCESSION   BBa_S03176
VERSION     BBa_S03176.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=BBa_E0020
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
     misc_feature    949..967
                     /label=TetR 1
     misc_feature    949..1002
                     /label=BBa_R0040
     promoter        968..973
                     /label=-35
     misc_feature    974..992
                     /label=TetR 2
     promoter        991..996
                     /label=-10
     promoter        949..1002
                     /label=BBa_R0040
     misc_feature    1015..1018
                     /label=conserved
     RBS             1011..1022
                     /label=BBa_B0034
     CDS             1029..1751
                     /label=BBa_E0030
     stem_loop       1771..1814
                     /label=stem_loop
     misc_feature    1760..1839
                     /label=BBa_B0010
     terminator      1760..1839
                     /label=BBa_B0010
     stem_loop       1855..1874
                     /label=T7 TE
     misc_feature    1881..1881
                     /label=stop
     polyA_site      1875..1888
                     /label=polya
     misc_feature    1848..1888
                     /label=BBa_B0012
     terminator      1848..1888
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc cctatcagtg
      961 atagagattg acatccctat cagtgataga gatactgagc actactagag aaagaggaga
     1021 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1081 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1141 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1201 ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc
     1261 acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1321 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1381 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1441 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1501 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1561 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1621 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1681 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1741 acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1801 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1861 ccttcgggtg ggcctttctg cgtttata
//