LOCUS       BBa_S03181              1804 bp    DNA     linear       16-Feb-2025
DEFINITION  I0461.R0040.I0462
ACCESSION   BBa_S03181
VERSION     BBa_S03181.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    598..629
                     /label=LVA
     CDS             19..597
                     /label=luxI
     misc_feature    19..636
                     /label=BBa_C0061
     misc_feature    637..661
                     /label=Help:Barcodes
     CDS             19..636
                     /label=BBa_C0061
     stem_loop       681..724
                     /label=stem_loop
     misc_feature    670..749
                     /label=BBa_B0010
     terminator      670..749
                     /label=BBa_B0010
     stem_loop       765..784
                     /label=T7 TE
     misc_feature    791..791
                     /label=stop
     polyA_site      785..798
                     /label=polya
     misc_feature    758..798
                     /label=BBa_B0012
     terminator      758..798
                     /label=BBa_B0012
     misc_feature    807..825
                     /label=TetR 1
     misc_feature    807..860
                     /label=BBa_R0040
     promoter        826..831
                     /label=-35
     misc_feature    832..850
                     /label=TetR 2
     promoter        849..854
                     /label=-10
     promoter        807..860
                     /label=BBa_R0040
     misc_feature    873..876
                     /label=conserved
     RBS             869..880
                     /label=BBa_B0034
     misc_feature    887..888
                     /label=prefix
     misc_feature    1060..1060
                     /label=T
     misc_feature    1378..1378
                     /label=A
     CDS             887..1636
                     /label=luxR
     misc_feature    887..1642
                     /label=BBa_C0062
     misc_feature    1643..1667
                     /label=Help:Barcodes
     CDS             887..1642
                     /label=BBa_C0062
     stem_loop       1687..1730
                     /label=stem_loop
     misc_feature    1676..1755
                     /label=BBa_B0010
     terminator      1676..1755
                     /label=BBa_B0010
     stem_loop       1771..1790
                     /label=T7 TE
     misc_feature    1797..1797
                     /label=stop
     polyA_site      1791..1804
                     /label=polya
     misc_feature    1764..1804
                     /label=BBa_B0012
     terminator      1764..1804
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca
       61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag
      121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa
      181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca
      241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc
      301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata
      361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt
      421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag
      481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact
      541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct
      601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct
      661 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      721 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      781 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca
      841 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatga aaaacataaa
      901 tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat
      961 taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat
     1021 catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa
     1081 atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag tagattattc
     1141 taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa
     1201 atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc
     1261 tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa
     1321 ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct
     1381 agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa
     1441 aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa
     1501 aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact
     1561 caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg
     1621 cccatacttt aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg
     1681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1741 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1801 tata
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