##gff-version 3
##sequence-region BBa_S03181 1 1804
BBa_S03181	.	ribosome_entry_site	869	880	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03181	.	conserved_region	873	876	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03181	.	terminator	670	749	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03181	.	stem_loop	681	724	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03181	.	engineered_region	670	749	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03181	.	CDS	887	1642	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S03181	.	CDS	887	1636	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S03181	.	engineered_region	887	1642	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S03181	.	engineered_region	887	888	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S03181	.	point_mutation	1060	1060	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S03181	.	engineered_tag	1643	1667	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S03181	.	point_mutation	1378	1378	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S03181	.	terminator	1764	1804	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03181	.	stem_loop	1771	1790	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03181	.	stop_codon	1797	1797	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03181	.	engineered_region	1764	1804	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03181	.	polyA_site	1791	1804	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03181	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03181	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03181	.	terminator	1676	1755	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03181	.	stem_loop	1687	1730	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03181	.	engineered_region	1676	1755	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03181	.	terminator	758	798	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03181	.	stem_loop	765	784	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03181	.	stop_codon	791	791	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03181	.	engineered_region	758	798	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03181	.	polyA_site	785	798	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03181	.	promoter	807	860	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S03181	.	non_covalent_binding_site	807	825	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03181	.	engineered_region	807	860	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03181	.	promoter	826	831	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03181	.	promoter	849	854	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03181	.	non_covalent_binding_site	832	850	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03181	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S03181	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S03181	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S03181	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S03181	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
>BBa_S03181
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaga
aagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaag
caataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttat
cctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgcta
atttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaa
tgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctatt
catacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttac
atgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatc
aaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaa
atattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgcc
aaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtg
tagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata