##gff-version 3
##sequence-region BBa_S03183 1 613
BBa_S03183	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_S03183	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S03183	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S03183	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S03183	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S03183	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S03183	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S03183	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S03183	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S03183	.	engineered_region	106	613	.	+	0	ID=BBa_E0433;Name=BBa_E0433
BBa_S03183	.	engineered_region	124	476	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_E0433
BBa_S03183	.	promoter	296	314	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_S03183	.	sequence_alteration	201	201	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_S03183	.	sequence_alteration	234	234	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_S03183	.	promoter	149	158	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_S03183	.	sequence_alteration	204	204	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_S03183	.	sequence_alteration	231	231	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_S03183	.	sequence_alteration	210	210	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_S03183	.	CDS	124	471	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_S03183	.	sequence_feature	322	471	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_S03183	.	sequence_feature	124	138	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_S03183	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0433
BBa_S03183	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03183	.	terminator	485	613	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0433
BBa_S03183	.	terminator	485	564	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S03183	.	engineered_region	485	564	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03183	.	stem_loop	496	539	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03183	.	terminator	573	613	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S03183	.	stop_codon	606	606	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03183	.	polyA_site	600	613	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03183	.	stem_loop	580	599	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03183	.	engineered_region	573	613	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S03183
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccct
cactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaat
tcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacg
cgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcaca
tccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata