LOCUS       BBa_S03186              2668 bp    DNA     linear       17-May-2024
DEFINITION  R0011.B0034.C0051.I0461.R0040.I0462
ACCESSION   BBa_S03186
VERSION     BBa_S03186.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..856
                     /label=BBa_C0051
     misc_feature    869..872
                     /label=conserved
     RBS             865..876
                     /label=BBa_B0034
     misc_feature    1462..1493
                     /label=LVA
     CDS             883..1461
                     /label=luxI
     misc_feature    883..1500
                     /label=BBa_C0061
     misc_feature    1501..1525
                     /label=Help:Barcodes
     CDS             883..1500
                     /label=BBa_C0061
     stem_loop       1545..1588
                     /label=stem_loop
     misc_feature    1534..1613
                     /label=BBa_B0010
     terminator      1534..1613
                     /label=BBa_B0010
     stem_loop       1629..1648
                     /label=T7 TE
     misc_feature    1655..1655
                     /label=stop
     polyA_site      1649..1662
                     /label=polya
     misc_feature    1622..1662
                     /label=BBa_B0012
     terminator      1622..1662
                     /label=BBa_B0012
     misc_feature    865..1662
                     /label=BBa_F1610
     misc_feature    1671..1689
                     /label=TetR 1
     misc_feature    1671..1724
                     /label=BBa_R0040
     promoter        1690..1695
                     /label=-35
     misc_feature    1696..1714
                     /label=TetR 2
     promoter        1713..1718
                     /label=-10
     promoter        1671..1724
                     /label=BBa_R0040
     misc_feature    1737..1740
                     /label=conserved
     RBS             1733..1744
                     /label=BBa_B0034
     misc_feature    1751..1752
                     /label=prefix
     misc_feature    1924..1924
                     /label=T
     misc_feature    2242..2242
                     /label=A
     CDS             1751..2500
                     /label=luxR
     misc_feature    1751..2506
                     /label=BBa_C0062
     misc_feature    2507..2531
                     /label=Help:Barcodes
     CDS             1751..2506
                     /label=BBa_C0062
     stem_loop       2551..2594
                     /label=stem_loop
     misc_feature    2540..2619
                     /label=BBa_B0010
     terminator      2540..2619
                     /label=BBa_B0010
     stem_loop       2635..2654
                     /label=T7 TE
     misc_feature    2661..2661
                     /label=stop
     polyA_site      2655..2668
                     /label=polya
     misc_feature    2628..2668
                     /label=BBa_B0012
     terminator      2628..2668
                     /label=BBa_B0012
     misc_feature    1733..2668
                     /label=BBa_I0462
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagaaagag gagaaatact agatgactat aatgataaaa
      901 aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat
      961 caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat
     1021 gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt
     1081 ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa
     1141 ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct
     1201 gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt
     1261 gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca
     1321 acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa
     1381 gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag
     1441 tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa
     1501 ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc
     1561 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1621 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag
     1681 tgatagagat tgacatccct atcagtgata gagatactga gcactactag agaaagagga
     1741 gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa
     1801 agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca
     1861 ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc
     1921 aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa
     1981 atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt
     2041 tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg
     2101 tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag
     2161 ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa
     2221 cataccatta attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa
     2281 taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg
     2341 aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca
     2401 tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc
     2461 aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta
     2521 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2581 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2641 ccttcgggtg ggcctttctg cgtttata
//