##gff-version 3
##sequence-region BBa_S03186 1 2668
BBa_S03186	.	promoter	1671	1724	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S03186	.	non_covalent_binding_site	1696	1714	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03186	.	promoter	1690	1695	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03186	.	engineered_region	1671	1724	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03186	.	non_covalent_binding_site	1671	1689	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03186	.	promoter	1713	1718	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03186	.	CDS	82	856	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_S03186	.	tag	793	825	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S03186	.	CDS	85	792	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S03186	.	engineered_tag	832	856	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S03186	.	engineered_region	865	1662	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_S03186	.	terminator	1622	1662	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_S03186	.	engineered_region	1622	1662	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03186	.	stop_codon	1655	1655	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03186	.	stem_loop	1629	1648	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03186	.	polyA_site	1649	1662	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03186	.	CDS	883	1500	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_S03186	.	engineered_region	883	1500	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S03186	.	engineered_tag	1501	1525	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S03186	.	tag	1462	1493	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S03186	.	CDS	883	1461	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S03186	.	terminator	1534	1613	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_S03186	.	stem_loop	1545	1588	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03186	.	engineered_region	1534	1613	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03186	.	ribosome_entry_site	865	876	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_S03186	.	conserved_region	869	872	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03186	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S03186	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S03186	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S03186	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S03186	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S03186	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S03186	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03186	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03186	.	engineered_region	1733	2668	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_S03186	.	terminator	2540	2619	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_S03186	.	stem_loop	2551	2594	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03186	.	engineered_region	2540	2619	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03186	.	terminator	2628	2668	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_S03186	.	engineered_region	2628	2668	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03186	.	stop_codon	2661	2661	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03186	.	stem_loop	2635	2654	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03186	.	polyA_site	2655	2668	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03186	.	CDS	1751	2506	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_S03186	.	engineered_region	1751	2506	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S03186	.	CDS	1751	2500	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S03186	.	point_mutation	1924	1924	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S03186	.	point_mutation	2242	2242	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S03186	.	engineered_tag	2507	2531	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S03186	.	engineered_region	1751	1752	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S03186	.	ribosome_entry_site	1733	1744	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_S03186	.	conserved_region	1737	1740	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03186
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcc
cttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaaga
ggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattcta
agtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatg
ataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaac
aggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaa
ttaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaag
ctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaa
gcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtct
atgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataac
tctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagag
gagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaata
atgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctca
ttctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaattta
ataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctg
taaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatac
ggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcg
tgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaaca
acgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatatt
aggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagt
atttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagat
cactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata