LOCUS BBa_S03187 1978 bp DNA linear 25-Nov-2024 DEFINITION B0034.C0051.B0034.C0061.E0433 ACCESSION BBa_S03187 VERSION BBa_S03187.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..729 /label=cI lambda misc_feature 730..762 /label=LVA misc_feature 769..793 /label=Help:Barcodes CDS 19..793 /label=BBa_C0051 misc_feature 806..809 /label=conserved RBS 802..813 /label=BBa_B0034 misc_feature 1399..1430 /label=LVA CDS 820..1398 /label=luxI misc_feature 820..1437 /label=BBa_C0061 misc_feature 1438..1462 /label=Help:Barcodes CDS 820..1437 /label=BBa_C0061 misc_feature 1475..1478 /label=conserved RBS 1471..1482 /label=BBa_B0034 CDS 1489..1836 /label=lacZ alpha misc_feature 1569..1569 /label=T misc_feature 1575..1575 /label=A misc_feature 1596..1596 /label=C misc_feature 1566..1566 /label=C misc_feature 1599..1599 /label=G promoter 1514..1523 /label=T3 promoter promoter 1661..1679 /label=T7 promoter misc_feature 1687..1836 /label=lacZ gene fragment misc_feature 1489..1503 /label=lacZ gene fragment misc_feature 1489..1841 /label=BBa_E0033 stem_loop 1861..1904 /label=stem_loop misc_feature 1850..1929 /label=BBa_B0010 terminator 1850..1929 /label=BBa_B0010 stem_loop 1945..1964 /label=T7 TE misc_feature 1971..1971 /label=stop polyA_site 1965..1978 /label=polya misc_feature 1938..1978 /label=BBa_B0012 terminator 1938..1978 /label=BBa_B0012 terminator 1850..1978 /label=BBa_B0015 misc_feature 1471..1978 /label=BBa_E0433 ORIGIN 1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 781 tagtgtagat cgctactaga gaaagaggag aaatactaga tgactataat gataaaaaaa 841 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa 901 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag 961 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga 1021 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg 1081 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta 1141 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa 1201 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca 1261 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa 1321 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt 1381 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 1441 tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat gaccatgatt 1501 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg 1561 gcggcagcac tagagctagt ggatcccccg ggctgtagaa attcgatatc aagcttatcg 1621 ataccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac 1681 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 1741 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 1801 accgatcgcc cttcccaaca gttgcgcagc ctgaataata atactagagc caggcatcaa 1861 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 1921 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //