LOCUS       BBa_S03254              1889 bp    DNA     linear       29-Nov-2024
DEFINITION  Intermediate part from assembly 317
ACCESSION   BBa_S03254
VERSION     BBa_S03254.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..804
                     /label=BBa_E0020
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    64..941
                     /label=BBa_E0420
     misc_feature    1..941
                     /label=BBa_I13601
     misc_feature    950..968
                     /label=TetR 1
     misc_feature    950..1003
                     /label=BBa_R0040
     promoter        969..974
                     /label=-35
     misc_feature    975..993
                     /label=TetR 2
     promoter        992..997
                     /label=-10
     promoter        950..1003
                     /label=BBa_R0040
     misc_feature    1016..1019
                     /label=conserved
     RBS             1012..1023
                     /label=BBa_B0034
     CDS             1030..1752
                     /label=BBa_E0030
     stem_loop       1772..1815
                     /label=stem_loop
     misc_feature    1761..1840
                     /label=BBa_B0010
     terminator      1761..1840
                     /label=BBa_B0010
     stem_loop       1856..1875
                     /label=T7 TE
     misc_feature    1882..1882
                     /label=stop
     polyA_site      1876..1889
                     /label=polya
     misc_feature    1849..1889
                     /label=BBa_B0012
     terminator      1849..1889
                     /label=BBa_B0012
     misc_feature    950..1889
                     /label=BBa_I6032
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc
      301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc
      541 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt
      961 gatagagatt gacatcccta tcagtgatag agatactgag cactactaga gaaagaggag
     1021 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1081 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1141 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1201 tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac
     1261 cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1321 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1381 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1441 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1501 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1561 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1621 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1681 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1741 tacaagtaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1801 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1861 accttcgggt gggcctttct gcgtttata
//