LOCUS       BBa_S03258              1946 bp    DNA     linear       29-Nov-2024
DEFINITION  Intermediate part from assembly 317
ACCESSION   BBa_S03258
VERSION     BBa_S03258.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..798
                     /label=BBa_E0030
     stem_loop       818..861
                     /label=stem_loop
     misc_feature    807..886
                     /label=BBa_B0010
     terminator      807..886
                     /label=BBa_B0010
     stem_loop       902..921
                     /label=T7 TE
     misc_feature    928..928
                     /label=stop
     polyA_site      922..935
                     /label=polya
     misc_feature    895..935
                     /label=BBa_B0012
     terminator      895..935
                     /label=BBa_B0012
     misc_feature    1..935
                     /label=BBa_I6036
     misc_feature    946..962
                     /label=lac O1
     promoter        963..968
                     /label=-35
     misc_feature    969..985
                     /label=lac O1
     promoter        986..991
                     /label=-10
     misc_feature    944..997
                     /label=BBa_R0011
     promoter        944..997
                     /label=BBa_R0011
     misc_feature    1011..1014
                     /label=conserved
     RBS             1007..1018
                     /label=BBa_B0034
     CDS             1025..1747
                     /label=BBa_E0020
     stem_loop       1767..1810
                     /label=stem_loop
     misc_feature    1756..1835
                     /label=BBa_B0010
     terminator      1756..1835
                     /label=BBa_B0010
     stem_loop       1851..1870
                     /label=T7 TE
     misc_feature    1877..1877
                     /label=stop
     polyA_site      1871..1884
                     /label=polya
     misc_feature    1844..1884
                     /label=BBa_B0012
     terminator      1844..1884
                     /label=BBa_B0012
     misc_feature    1007..1884
                     /label=BBa_E0420
     misc_feature    944..1884
                     /label=BBa_I13601
     misc_feature    1893..1911
                     /label=TetR 1
     misc_feature    1893..1946
                     /label=BBa_R0040
     promoter        1912..1917
                     /label=-35
     misc_feature    1918..1936
                     /label=TetR 2
     promoter        1935..1940
                     /label=-10
     promoter        1893..1946
                     /label=BBa_R0040
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac
      301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      901 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac
      961 aattgacatt gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata
     1021 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1081 ggacggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1141 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1201 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
     1261 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1321 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1381 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1441 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa
     1501 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1561 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1621 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1681 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1741 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1801 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1861 cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc
     1921 ctatcagtga tagagatact gagcac
//