LOCUS       BBa_S03263              1177 bp    DNA     linear       22-Nov-2024
DEFINITION  Intermediate part from assembly 317
ACCESSION   BBa_S03263
VERSION     BBa_S03263.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    1010..1015
                     /label=2
     misc_feature    977..1009
                     /label=LVA
     CDS             227..976
                     /label=aiiA
     misc_feature    227..1015
                     /label=BBa_C0060
     misc_feature    1016..1040
                     /label=Help:Barcodes
     CDS             227..1015
                     /label=BBa_C0060
     stem_loop       1060..1103
                     /label=stem_loop
     misc_feature    1049..1128
                     /label=BBa_B0010
     terminator      1049..1128
                     /label=BBa_B0010
     stem_loop       1144..1163
                     /label=T7 TE
     misc_feature    1170..1170
                     /label=stop
     polyA_site      1164..1177
                     /label=polya
     misc_feature    1137..1177
                     /label=BBa_B0012
     terminator      1137..1177
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga cagtaaagaa
      241 gctttatttc gtcccagcag gtcgttgtat gttggatcat tcgtctgtta atagtacatt
      301 aacaccagga gaattattag acttaccggt ttggtgttat cttttggaga ctgaagaagg
      361 acctatttta gtagatacag gtatgccaga aagtgcagtt aataatgaag gtctttttaa
      421 cggtacattt gtcgaagggc aggttttacc gaaaatgact gaagaagata gaatcgtgaa
      481 tattttaaaa cgggttggtt atgagccgga agaccttctt tatattatta gttctcactt
      541 gcattttgat catgcaggag gaaatggcgc ttttataaat acaccaatca ttgtacagcg
      601 tgctgaatat gaggcggcgc agcatagcga agaatatttg aaagaatgta tattgccgaa
      661 tttaaactac aaaatcattg aaggtgatta tgaagtcgta ccaggagttc aattattgca
      721 tacaccaggc catactccag ggcatcaatc gctattaatt gagacagaaa aatccggtcc
      781 tgtattatta acgattgatg catcgtatac gaaagagaat tttgaaaatg aagtgccatt
      841 tgcgggattt gattcagaat tagctttatc ttcaattaaa cgtttaaaag aagtggtgat
      901 gaaagagaag ccgattgttt tctttggaca tgatatagag caggaaaggg gatgtaaagt
      961 gttccctgaa tatatagctg caaacgacga aaactacgct ttagtagctt aataacgctg
     1021 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1081 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1141 actggctcac cttcgggtgg gcctttctgc gtttata
//