LOCUS       BBa_S03264              1928 bp    DNA     linear       29-Nov-2024
DEFINITION  Intermediate part from assembly 317
ACCESSION   BBa_S03264
VERSION     BBa_S03264.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..842
                     /label=YFP (LVA)
     misc_feature    837..842
                     /label=2
     misc_feature    798..836
                     /label=SsrA
     misc_feature    322..322
                     /label=A (Q->L)
     misc_feature    81..842
                     /label=BBa_E0032
     misc_feature    81..842
                     /label=BBa_E0032
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    1..979
                     /label=BBa_I6062
     misc_feature    990..1006
                     /label=lac O1
     promoter        1007..1012
                     /label=-35
     misc_feature    1013..1029
                     /label=lac O1
     promoter        1030..1035
                     /label=-10
     misc_feature    988..1041
                     /label=BBa_R0011
     promoter        988..1041
                     /label=BBa_R0011
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1069..1791
                     /label=BBa_E0020
     stem_loop       1811..1854
                     /label=stem_loop
     misc_feature    1800..1879
                     /label=BBa_B0010
     terminator      1800..1879
                     /label=BBa_B0010
     stem_loop       1895..1914
                     /label=T7 TE
     misc_feature    1921..1921
                     /label=stop
     polyA_site      1915..1928
                     /label=polya
     misc_feature    1888..1928
                     /label=BBa_B0012
     terminator      1888..1928
                     /label=BBa_B0012
     misc_feature    1051..1928
                     /label=BBa_E0420
     misc_feature    988..1928
                     /label=BBa_I13601
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc
      301 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      541 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc
     1021 ggataacaag atactgagca catactagag aaagaggaga aatactagat ggtgagcaag
     1081 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac
     1141 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1201 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1261 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1321 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1381 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1441 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1501 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1561 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1621 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1681 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1741 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc
     1801 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1861 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1921 cgtttata
//