LOCUS BBa_S03266 5338 bp DNA linear 22-Nov-2024 DEFINITION Intermediate part from assembly 317 ACCESSION BBa_S03266 VERSION BBa_S03266.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 CDS 127..743 /label=tetR misc_feature 744..777 /label=SsrA misc_feature 784..808 /label=Help:Barcodes CDS 124..808 /label=BBa_C0040 stem_loop 828..871 /label=stem_loop misc_feature 817..896 /label=BBa_B0010 terminator 817..896 /label=BBa_B0010 stem_loop 912..931 /label=T7 TE misc_feature 938..938 /label=stop polyA_site 932..945 /label=polya misc_feature 905..945 /label=BBa_B0012 terminator 905..945 /label=BBa_B0012 terminator 817..945 /label=BBa_B0015 misc_feature 954..972 /label=TetR 1 misc_feature 954..1007 /label=BBa_R0040 promoter 973..978 /label=-35 misc_feature 979..997 /label=TetR 2 promoter 996..1001 /label=-10 promoter 954..1007 /label=BBa_R0040 misc_feature 1020..1023 /label=conserved RBS 1016..1027 /label=BBa_B0034 misc_feature 2123..2161 /label=LVA CDS 1034..2161 /label=lacI-LVA misc_feature 1034..2161 /label=BBa_C0012 misc_feature 2162..2186 /label=Help:Barcodes CDS 1034..2161 /label=BBa_C0012 stem_loop 2206..2249 /label=stem_loop misc_feature 2195..2274 /label=BBa_B0010 terminator 2195..2274 /label=BBa_B0010 stem_loop 2290..2309 /label=T7 TE misc_feature 2316..2316 /label=stop polyA_site 2310..2323 /label=polya misc_feature 2283..2323 /label=BBa_B0012 terminator 2283..2323 /label=BBa_B0012 terminator 2195..2323 /label=BBa_B0015 misc_feature 2334..2350 /label=lac O1 promoter 2351..2356 /label=-35 misc_feature 2357..2373 /label=lac O1 promoter 2374..2379 /label=-10 misc_feature 2332..2385 /label=BBa_R0011 promoter 2332..2385 /label=BBa_R0011 misc_feature 1016..2386 /label=BBa_Q04121 misc_feature 2399..2402 /label=conserved RBS 2395..2406 /label=BBa_B0034 CDS 2416..3123 /label=cI lambda misc_feature 3124..3156 /label=LVA misc_feature 3163..3187 /label=Help:Barcodes CDS 2413..3162 /label=BBa_C0051 stem_loop 3207..3250 /label=stem_loop misc_feature 3196..3275 /label=BBa_B0010 terminator 3196..3275 /label=BBa_B0010 stem_loop 3291..3310 /label=T7 TE misc_feature 3317..3317 /label=stop polyA_site 3311..3324 /label=polya misc_feature 3284..3324 /label=BBa_B0012 terminator 3284..3324 /label=BBa_B0012 misc_feature 3333..3429 /label=BBa_R0065 misc_feature 3338..3357 /label=Lux Box promoter 3379..3384 /label=-10 misc_feature 3389..3405 /label=OR2 cI promoter 3390..3390 /label=lux p(R) start promoter 3403..3408 /label=-35 misc_feature 3413..3429 /label=OR1 cI promoter 3426..3429 /label=-10 promoter 3333..3429 /label=BBa_R0065 misc_feature 2395..3429 /label=BBa_Q04511 misc_feature 3442..3445 /label=conserved RBS 3438..3449 /label=BBa_B0034 misc_feature 4035..4066 /label=LVA CDS 3456..4034 /label=luxI misc_feature 3456..4073 /label=BBa_C0061 misc_feature 4074..4098 /label=Help:Barcodes CDS 3456..4073 /label=BBa_C0061 stem_loop 4118..4161 /label=stem_loop misc_feature 4107..4186 /label=BBa_B0010 terminator 4107..4186 /label=BBa_B0010 stem_loop 4202..4221 /label=T7 TE misc_feature 4228..4228 /label=stop polyA_site 4222..4235 /label=polya misc_feature 4195..4235 /label=BBa_B0012 terminator 4195..4235 /label=BBa_B0012 terminator 4107..4235 /label=BBa_B0015 misc_feature 4244..4263 /label=LuxR/HSL promoter 4358..4365 /label=-10 promoter 4332..4337 /label=-35 misc_feature 4371..4371 /label=start misc_feature 4373..4392 /label=Putative LuxR/HSL misc_feature 4244..4394 /label=BBa_R0063 promoter 4244..4394 /label=BBa_R0063 misc_feature 4407..4410 /label=conserved RBS 4403..4414 /label=BBa_B0034 misc_feature 4421..4422 /label=prefix misc_feature 4594..4594 /label=T misc_feature 4912..4912 /label=A CDS 4421..5170 /label=luxR misc_feature 4421..5176 /label=BBa_C0062 misc_feature 5177..5201 /label=Help:Barcodes CDS 4421..5176 /label=BBa_C0062 stem_loop 5221..5264 /label=stem_loop misc_feature 5210..5289 /label=BBa_B0010 terminator 5210..5289 /label=BBa_B0010 stem_loop 5305..5324 /label=T7 TE misc_feature 5331..5331 /label=stop polyA_site 5325..5338 /label=polya misc_feature 5298..5338 /label=BBa_B0012 terminator 5298..5338 /label=BBa_B0012 terminator 5210..5338 /label=BBa_B0015 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag 181 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct 241 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg 301 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta 361 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta 421 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt 481 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat 541 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa 601 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac 661 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa 721 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa 781 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat 961 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga 1021 ggagaaatac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 1081 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 1141 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 1201 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 1261 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 1321 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 1381 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 1441 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 1501 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 1561 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 1621 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 1681 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 1741 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 1801 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 1861 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 1921 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 1981 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 2041 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 2101 ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata 2161 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct 2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2281 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag 2341 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagaaagag 2401 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca 2461 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct 2521 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat 2581 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa 2641 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg 2701 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca 2761 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa 2821 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc 2881 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag 2941 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc 3001 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc 3061 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt 3121 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt 3181 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3241 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3301 cgggtgggcc tttctgcgtt tatatactag agtaagcacc tgtaggatcg tacaggttta 3361 cgcaagaaaa tggtttgtta tagtcgaata acaccgtgcg tgttgactat tttacctctg 3421 gcggtgatat actagagaaa gaggagaaat actagatgac tataatgata aaaaaatcgg 3481 attttttggc aattccatcg gaggagtata aaggtattct aagtcttcgt tatcaagtgt 3541 ttaagcaaag acttgagtgg gacttagttg tagaaaataa ccttgaatca gatgagtatg 3601 ataactcaaa tgcagaatat atttatgctt gtgatgatac tgaaaatgta agtggatgct 3661 ggcgtttatt acctacaaca ggtgattata tgctgaaaag tgtttttcct gaattgcttg 3721 gtcaacagag tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt gctgtaggta 3781 aaaatagctc aaagataaat aactctgcta gtgaaattac aatgaaacta tttgaagcta 3841 tatataaaca cgctgttagt caaggtatta cagaatatgt aacagtaaca tcaacagcaa 3901 tagagcgatt tttaaagcgt attaaagttc cttgtcatcg tattggagac aaagaaattc 3961 atgtattagg tgatactaaa tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa 4021 aagcagtctt aaatgctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 4081 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 4141 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 4201 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta cgatcctaca 4261 ggtgcttatg ttaagtaatt gtattcccag cgatacaata gtgtgacaaa aatccaattt 4321 attagaatca aatgtcaatc cattaccgtt ttaatgatat ataacacgca aaacttgcga 4381 caaacaatag gtaatactag agaaagagga gaaatactag atgaaaaaca taaatgccga 4441 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca 4501 atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta 4561 tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag 4621 gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc 4681 caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc 4741 aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca 4801 tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat 4861 agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga 4921 taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga 4981 aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt 5041 aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac 5101 aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata 5161 ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 5221 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 5281 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //