LOCUS BBa_S03269 5248 bp DNA linear 26-Dec-2024 DEFINITION Intermediate part from assembly 317 ACCESSION BBa_S03269 VERSION BBa_S03269.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..741 /label=BBa_C0040 stem_loop 786..829 /label=stem_loop misc_feature 775..854 /label=BBa_B0010 terminator 775..854 /label=BBa_B0010 stem_loop 870..889 /label=T7 TE misc_feature 896..896 /label=stop polyA_site 890..903 /label=polya misc_feature 863..903 /label=BBa_B0012 terminator 863..903 /label=BBa_B0012 misc_feature 912..930 /label=TetR 1 misc_feature 912..965 /label=BBa_R0040 promoter 931..936 /label=-35 misc_feature 937..955 /label=TetR 2 promoter 954..959 /label=-10 promoter 912..965 /label=BBa_R0040 misc_feature 64..965 /label=BBa_Q04400 misc_feature 978..981 /label=conserved RBS 974..985 /label=BBa_B0034 misc_feature 2081..2119 /label=LVA CDS 992..2119 /label=lacI-LVA misc_feature 992..2119 /label=BBa_C0012 misc_feature 2120..2144 /label=Help:Barcodes CDS 992..2119 /label=BBa_C0012 stem_loop 2164..2207 /label=stem_loop misc_feature 2153..2232 /label=BBa_B0010 terminator 2153..2232 /label=BBa_B0010 stem_loop 2248..2267 /label=T7 TE misc_feature 2274..2274 /label=stop polyA_site 2268..2281 /label=polya misc_feature 2241..2281 /label=BBa_B0012 terminator 2241..2281 /label=BBa_B0012 terminator 2153..2281 /label=BBa_B0015 misc_feature 2292..2308 /label=lac O1 promoter 2309..2314 /label=-35 misc_feature 2315..2331 /label=lac O1 promoter 2332..2337 /label=-10 misc_feature 2290..2343 /label=BBa_R0011 promoter 2290..2343 /label=BBa_R0011 misc_feature 974..2344 /label=BBa_Q04121 misc_feature 2357..2360 /label=conserved RBS 2353..2364 /label=BBa_B0034 CDS 2374..3081 /label=cI lambda misc_feature 3082..3114 /label=LVA misc_feature 3121..3145 /label=Help:Barcodes CDS 2371..3120 /label=BBa_C0051 stem_loop 3165..3208 /label=stem_loop misc_feature 3154..3233 /label=BBa_B0010 terminator 3154..3233 /label=BBa_B0010 stem_loop 3249..3268 /label=T7 TE misc_feature 3275..3275 /label=stop polyA_site 3269..3282 /label=polya misc_feature 3242..3282 /label=BBa_B0012 terminator 3242..3282 /label=BBa_B0012 promoter 3328..3333 /label=-10 promoter 3305..3310 /label=-35 misc_feature 3315..3331 /label=OR1 misc_feature 3291..3307 /label=OR2 misc_feature 3291..3339 /label=BBa_R0051 promoter 3291..3339 /label=BBa_R0051 misc_feature 2353..3339 /label=BBa_Q04510 misc_feature 3352..3355 /label=conserved RBS 3348..3359 /label=BBa_B0034 misc_feature 3945..3976 /label=LVA CDS 3366..3944 /label=luxI misc_feature 3366..3983 /label=BBa_C0061 misc_feature 3984..4008 /label=Help:Barcodes CDS 3366..3983 /label=BBa_C0061 stem_loop 4028..4071 /label=stem_loop misc_feature 4017..4096 /label=BBa_B0010 terminator 4017..4096 /label=BBa_B0010 stem_loop 4112..4131 /label=T7 TE misc_feature 4138..4138 /label=stop polyA_site 4132..4145 /label=polya misc_feature 4105..4145 /label=BBa_B0012 terminator 4105..4145 /label=BBa_B0012 terminator 4017..4145 /label=BBa_B0015 misc_feature 4154..4173 /label=LuxR/HSL promoter 4268..4275 /label=-10 promoter 4242..4247 /label=-35 misc_feature 4281..4281 /label=start misc_feature 4283..4302 /label=Putative LuxR/HSL misc_feature 4154..4304 /label=BBa_R0063 promoter 4154..4304 /label=BBa_R0063 misc_feature 4317..4320 /label=conserved RBS 4313..4324 /label=BBa_B0034 misc_feature 4331..4332 /label=prefix misc_feature 4504..4504 /label=T misc_feature 4822..4822 /label=A CDS 4331..5080 /label=luxR misc_feature 4331..5086 /label=BBa_C0062 misc_feature 5087..5111 /label=Help:Barcodes CDS 4331..5086 /label=BBa_C0062 stem_loop 5131..5174 /label=stem_loop misc_feature 5120..5199 /label=BBa_B0010 terminator 5120..5199 /label=BBa_B0010 stem_loop 5215..5234 /label=T7 TE misc_feature 5241..5241 /label=stop polyA_site 5235..5248 /label=polya misc_feature 5208..5248 /label=BBa_B0012 terminator 5208..5248 /label=BBa_B0012 terminator 5120..5248 /label=BBa_B0015 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 961 agcactacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata 1021 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 1081 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 1141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 1201 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc 1261 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg 1321 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 1381 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 1441 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 1501 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 1561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg 1621 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca 1681 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga 1741 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 1801 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac 1861 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 1921 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 1981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 2041 gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 2161 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2221 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2281 atactagaga attgtgagcg gataacaatt gacattgtga gcggataaca agatactgag 2341 cacatactag agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa 2401 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt 2461 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct 2521 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc 2581 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa 2641 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt 2701 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg 2761 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc 2821 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac 2881 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt 2941 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag 3001 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag 3061 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa 3121 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc 3181 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3241 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg 3301 cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat 3361 actagatgac tataatgata aaaaaatcgg attttttggc aattccatcg gaggagtata 3421 aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg gacttagttg 3481 tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat atttatgctt 3541 gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca ggtgattata 3601 tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa gatcctaata 3661 tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc aaagataaat aactctgcta 3721 gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt caaggtatta 3781 cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt attaaagttc 3841 cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa tcggttgtat 3901 tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaatgctgca aacgacgaaa 3961 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag 4021 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 4081 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4141 ttatatacta gagacctgta cgatcctaca ggtgcttatg ttaagtaatt gtattcccag 4201 cgatacaata gtgtgacaaa aatccaattt attagaatca aatgtcaatc cattaccgtt 4261 ttaatgatat ataacacgca aaacttgcga caaacaatag gtaatactag agaaagagga 4321 gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa 4381 agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca 4441 ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc 4501 aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa 4561 atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt 4621 tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg 4681 tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag 4741 ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa 4801 cataccatta attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa 4861 taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg 4921 aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca 4981 tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc 5041 aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta 5101 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 5161 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 5221 ccttcgggtg ggcctttctg cgtttata //