BBa_I12007
1
Prm +
Modified lambda Prm promoter (OR-3 obliterated)
2004-07-14T11:00:00Z
2015-08-31T04:07:31Z
Shih and Gussin (1983)
Released HQ 2013
Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
false
true
_3_
0
103
7
In stock
false
In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
true
Hans
annotation937702
1
OR2
range937702
1
33
49
annotation937703
1
-35
range937703
1
48
53
annotation937705
1
-10
range937705
1
71
76
annotation937704
1
mutate to obliterate OR3
range937704
1
59
65
annotation937701
1
OR1
range937701
1
9
25
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_S03311
1
BBa_S03311
I12007.B0032
2005-08-22T11:00:00Z
2015-05-08T01:14:21Z
Assembly for J11005 by Lucy and Randy 8-05
false
false
_42_1_
0
25
1
Not in stock
false
false
Randy Rettberg
component1668026
1
BBa_B0032
component1668018
1
BBa_I12007
annotation1668026
1
BBa_B0032
range1668026
1
91
103
annotation1668018
1
BBa_I12007
range1668018
1
1
82
BBa_I12007_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
BBa_B0032_sequence
1
tcacacaggaaag
BBa_S03311_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagtcacacaggaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z