BBa_I1031
1
BBa_I1031
CI(1) "KISS" asRNA
2003-01-31T12:00:00Z
2015-08-31T04:07:29Z
see references
Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1030</bb_part>'s mRNA transcript. Has LacO-1 regulatory region with promoter. Part of the XOR gate comprised of <bb_part>BBa_I1030</bb_part> and <bb_part>BBa_I1040</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1011</bb_part> and <bb_part>BBa_I1031</bb_part>).
false
false
_1_
0
24
7
It's complicated
false
<P> <P>Complementary to beginning of <bb_part>BBa_I1030</bb_part> transcript covering junk region, RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. [<A href="http://biobricks.ai.mit.edu/BB_References.htm#KEIL96">KEIL96</A>] <P> Incompatible with systems containing <bb_part>BBa_I1032</bb_part> <br>Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
false
Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
annotation7051
1
BBa_I1031
range7051
1
1
163
annotation1885
1
LacO-1
range1885
1
1
55
annotation1884
1
antisense region for I1030
range1884
1
56
163
BBa_S03324
1
BBa_S03324
--Specify Parts List--
2005-08-23T11:00:00Z
2015-05-08T01:14:21Z
R0010. I1031
true
false
_41_
0
386
41
Discontinued
false
false
Lucy Zhang
component1668672
1
BBa_I1031
component1668658
1
BBa_R0010
annotation1668658
1
BBa_R0010
range1668658
1
1
200
annotation1668672
1
BBa_I1031
range1668672
1
209
371
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_S03324_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtcgacatcgaaccggtttcctgtcgttgctgttgtgcaaagct
BBa_I1031_sequence
1
ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtcgacatcgaaccggtttcctgtcgttgctgttgtgcaaagct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z