LOCUS BBa_S03329 2125 bp DNA linear 10-May-2024 DEFINITION --Specify Parts List-- ACCESSION BBa_S03329 VERSION BBa_S03329.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1200 /label=BBa_R0065 misc_feature 1109..1128 /label=Lux Box promoter 1150..1155 /label=-10 misc_feature 1160..1176 /label=OR2 cI promoter 1161..1161 /label=lux p(R) start promoter 1174..1179 /label=-35 misc_feature 1184..1200 /label=OR1 cI promoter 1197..1200 /label=-10 promoter 1104..1200 /label=BBa_R0065 misc_feature 1213..1216 /label=conserved RBS 1209..1220 /label=BBa_B0034 misc_feature 1983..1988 /label=stop misc_feature 1944..1982 /label=AAV misc_feature 1227..1988 /label=BBa_E0034 stem_loop 2008..2051 /label=stem_loop misc_feature 1997..2076 /label=BBa_B0010 terminator 1997..2076 /label=BBa_B0010 stem_loop 2092..2111 /label=T7 TE misc_feature 2118..2118 /label=stop polyA_site 2112..2125 /label=polya misc_feature 2085..2125 /label=BBa_B0012 terminator 2085..2125 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagtaagcac ctgtaggatc gtacaggttt acgcaagaaa 1141 atggtttgtt atagtcgaat aacaccgtgc gtgttgacta ttttacctct ggcggtgata 1201 tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg 1261 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 1321 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 1381 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct 1441 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag 1501 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 1561 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 1621 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 1681 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 1741 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 1801 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc 1861 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 1921 tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctgctgctg 1981 tttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2041 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2101 tcgggtgggc ctttctgcgt ttata //