LOCUS       BBa_I7100                940 bp    DNA     linear       14-May-2024
DEFINITION  TetR repressible GFP generator
ACCESSION   BBa_I7100
VERSION     BBa_I7100.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..803
                     /label=GFP protein
     CDS             84..803
                     /label=BBa_E0040
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      121 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      181 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      241 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      301 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      361 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      421 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      481 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      541 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      781 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata
//
LOCUS       BBa_S03386               940 bp    DNA     linear       14-May-2024
DEFINITION  tetR GFP
ACCESSION   BBa_S03386
VERSION     BBa_S03386.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..803
                     /label=GFP protein
     CDS             84..803
                     /label=BBa_E0040
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      121 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      181 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      241 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      301 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      361 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      421 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      481 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      541 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      781 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata
//