LOCUS BBa_S03391 1077 bp DNA linear 23-Dec-2024 DEFINITION --Specify Parts List-- ACCESSION BBa_S03391 VERSION BBa_S03391.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 138..156 /label=TetR 1 misc_feature 138..191 /label=BBa_R0040 promoter 157..162 /label=-35 misc_feature 163..181 /label=TetR 2 promoter 180..185 /label=-10 promoter 138..191 /label=BBa_R0040 misc_feature 204..207 /label=conserved RBS 200..211 /label=BBa_B0034 CDS 218..940 /label=BBa_E0020 stem_loop 960..1003 /label=stem_loop misc_feature 949..1028 /label=BBa_B0010 terminator 949..1028 /label=BBa_B0010 stem_loop 1044..1063 /label=T7 TE misc_feature 1070..1070 /label=stop polyA_site 1064..1077 /label=polya misc_feature 1037..1077 /label=BBa_B0012 terminator 1037..1077 /label=BBa_B0012 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag 181 atactgagca ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct 241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 421 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 541 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 601 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 661 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 721 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 781 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 841 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 901 cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa 961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata // LOCUS BBa_J01051 1077 bp DNA linear 23-Dec-2024 DEFINITION [DblTerminator][OnCFP] ACCESSION BBa_J01051 VERSION BBa_J01051.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 138..156 /label=TetR 1 misc_feature 138..191 /label=BBa_R0040 promoter 157..162 /label=-35 misc_feature 163..181 /label=TetR 2 promoter 180..185 /label=-10 promoter 138..191 /label=BBa_R0040 misc_feature 204..207 /label=conserved RBS 200..211 /label=BBa_B0034 CDS 218..940 /label=BBa_E0020 stem_loop 960..1003 /label=stem_loop misc_feature 949..1028 /label=BBa_B0010 terminator 949..1028 /label=BBa_B0010 stem_loop 1044..1063 /label=T7 TE misc_feature 1070..1070 /label=stop polyA_site 1064..1077 /label=polya misc_feature 1037..1077 /label=BBa_B0012 terminator 1037..1077 /label=BBa_B0012 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag 181 atactgagca ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct 241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 421 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 541 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 601 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 661 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 721 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 781 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 841 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 901 cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa 961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //