##gff-version 3
##sequence-region BBa_S03415 1 3418
BBa_S03415	.	CDS	19	768	.	+	0	ID=BBa_I15009;Name=BBa_I15009
BBa_S03415	.	CDS	19	768	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_S03415	.	engineered_tag	769	793	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_S03415	.	terminator	3290	3369	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03415	.	engineered_region	3290	3369	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03415	.	stem_loop	3301	3344	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03415	.	terminator	890	930	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03415	.	stop_codon	923	923	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03415	.	polyA_site	917	930	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03415	.	stem_loop	897	916	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03415	.	engineered_region	890	930	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03415	.	promoter	939	992	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S03415	.	engineered_region	939	992	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03415	.	promoter	958	963	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03415	.	promoter	981	986	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03415	.	non_covalent_binding_site	939	957	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03415	.	non_covalent_binding_site	964	982	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03415	.	terminator	3378	3418	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03415	.	stop_codon	3411	3411	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03415	.	polyA_site	3405	3418	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03415	.	stem_loop	3385	3404	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03415	.	engineered_region	3378	3418	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03415	.	CDS	1019	3256	.	+	0	ID=BBa_I15010;Name=BBa_I15010
BBa_S03415	.	engineered_tag	3257	3281	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_S03415	.	CDS	1019	3256	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_S03415	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03415	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03415	.	terminator	802	881	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03415	.	engineered_region	802	881	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03415	.	stem_loop	813	856	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03415	.	ribosome_entry_site	1001	1012	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03415	.	conserved_region	1005	1008	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03415
aaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatga
ttcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttggg
ctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatg
catttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctac
ctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcga
tcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgccc
ccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaa
gggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagca
ccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggg
gaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagat
ctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcag
tgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggccacc
accgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacg
gtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatc
gccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacg
gccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacgggg
tttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcgg
tttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtc
gaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattg
ccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcg
gctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaacccc
agcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaata
tgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaac
ccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccag
gaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcgg
cggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctt
tggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgg
gaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtg
gcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattgggg
cggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctc
tggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcga
tcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgct
gctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcag
gatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgc
gcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggcta
tgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtg
gcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcct
ggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcga
cagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctg
gagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatga
caaaagaagggtaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata