LOCUS       BBa_S03422               992 bp    DNA     linear       17-May-2024
DEFINITION  --Specify Parts List--
ACCESSION   BBa_S03422
VERSION     BBa_S03422.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..768
                     /label=PcyA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..768
                     /label=BBa_I15009
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     misc_feature    939..957
                     /label=TetR 1
     misc_feature    939..992
                     /label=BBa_R0040
     promoter        958..963
                     /label=-35
     misc_feature    964..982
                     /label=TetR 2
     promoter        981..986
                     /label=-10
     promoter        939..992
                     /label=BBa_R0040
ORIGIN
        1 aaagaggaga aatactagat ggccgtcact gatttaagtt tgaccaattc ttccctgatg
       61 cctacgttga acccgatgat tcaacagttg gccctggcga tcgccgctag ttggcaaagt
      121 ttacccctca agccctatca attgccggag gatttgggct acgtagaagg ccgcctggaa
      181 ggggaaaagt tagtgattga aaatcggtgc taccaaacgc cccagtttcg caaaatgcat
      241 ttggagttgg ccaaggtggg caaagggttg gatattctcc actgtgtaat gtttcctgag
      301 cctttatacg gtctaccttt gtttggctgt gacattgtgg ccggccccgg tggagtaagt
      361 gcggctattg cggatctatc ccccacccaa agcgatcgcc aattgcccgc agcgtaccaa
      421 aaatcattgg cagagctagg ccagccagaa tttgagcaac aacgggaatt gcccccctgg
      481 ggagaaatat tttctgaata ttgtttattc atccgtccca gcaatgtcac tgaagaagaa
      541 agatttgtac aaagggtagt ggactttttg caaattcatt gtcaccaatc catcgttgcc
      601 gaacccttgt ctgaagctca aactttggag caccgtcagg ggcaaattca ttactgccaa
      661 caacaacaga aaaatgataa aacccgtcgg gtactggaaa aagcttttgg ggaagcttgg
      721 gcggaacggt atatgagcca agtcttattt gatgttatcc aataataact ctgatagtgc
      781 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg
      961 acatccctat cagtgataga gatactgagc ac
//