LOCUS       BBa_S03423              2480 bp    DNA     linear       17-May-2024
DEFINITION  --Specify Parts List--
ACCESSION   BBa_S03423
VERSION     BBa_S03423.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..156
                     /label=TetR 1
     misc_feature    138..191
                     /label=BBa_R0040
     promoter        157..162
                     /label=-35
     misc_feature    163..181
                     /label=TetR 2
     promoter        180..185
                     /label=-10
     promoter        138..191
                     /label=BBa_R0040
     misc_feature    204..207
                     /label=conserved
     RBS             200..211
                     /label=BBa_B0034
     CDS             218..2455
                     /label=Cph8
     misc_feature    2456..2480
                     /label=Help:Barcodes
     CDS             218..2455
                     /label=BBa_I15010
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
      181 atactgagca ctactagaga aagaggagaa atactagatg gccaccaccg tacaactcag
      241 cgaccaatcc ctccgtcagc tagaaaccct cgccatccac accgcccacc tgattcagcc
      301 ccacggttta gtggtggtcc tgcaagaacc agacctcacc atcagccaaa ttagcgccaa
      361 ctgcaccggc attttagggc gatcgccaga ggatttgttg ggcagaaccc taggggaagt
      421 gtttgatagc tttcagattg atcccatcca gagtcgccta acggccggac aaatcagcag
      481 cctcaacccc agtaaacttt gggcgcgggt catgggggac gactttgtca tttttgacgg
      541 ggtttttcat cgcaacagtg acggtttatt ggtatgtgaa ctcgagccag cctacacttc
      601 cgataatctg cccttcctcg gtttttatca catggccaac gctgccctga atcggttgcg
      661 ccaacaagct aatctacggg atttctacga tgttattgtc gaagaagtcc gccgtatgac
      721 tggctttgac cgggtgatgc tataccgctt tgatgaaaat aaccacggtg atgtcattgc
      781 cgaagataaa cgggatgata tggaacccta tttgggcctg cactatcccg aatcggatat
      841 tccccaaccc gcccgtcggc tatttatcca caaccccatt cgagtaattc ccgatgttta
      901 tggtgtggcg gtgcccctga ccccagcggt taaccccagc accaaccgag cggtggattt
      961 aacagaatcc attctgcgca gtgcgtacca ttgccacttg acctatctga aaaatatggg
     1021 ggtaggagcg tctttaacca tttccctaat taaggacggc catctctggg ggctcattgc
     1081 ctgccaccat caaaccccca aagtaattcc ctttgaactg cgtaaagcct gcgaattttt
     1141 tggtcgggtg gtgtttagca acatttccgc ccaggaagat acggaaacct tcgattaccg
     1201 ggtgcagctg gcggagcatg aagcggtttt attggacaaa atgaccacgg cggcggattt
     1261 tgtcgaagga ttaactaatc atcccgatcg cctgttggga ttaacgggct cccagggggc
     1321 ggccatttgc tttggggaaa aattgatttt agtaggggaa accccggacg agaaagcagt
     1381 gcaatattta ctgcaatggt tggagaatcg ggaagtgcaa gacgttttct tcacctcttc
     1441 cctctcacaa atttatcctg atgcagtgaa ttttaaatcc gtggccagtg gcttattggc
     1501 cattcccatt gcccgtcaca actttttgct ctggtttcgc cctgaagtgt tgcaaacggt
     1561 taattggggc ggtgacccaa atcatgctta cgaagctacc caggaagacg gtaaaatcga
     1621 gctccatccc cgccaatcct ttgacctctg gaaagaaatt gtccgactcc aatctttgcc
     1681 ctggcaatcg gtggaaatcc aaagtgccct ggccctgaaa aaggcgatcg tcaacctcat
     1741 tttgcgccag gcagaagaat tgcatatggc ggctggtgtt aagcaactgg cggatgaccg
     1801 cacgctgctg atggcggggg taagtcacga cttgcgcacg ccgctgacgc gtattcgcct
     1861 ggcgactgag atgatgagcg agcaggatgg ctatctggca gaatcgatca ataaagatat
     1921 cgaagagtgc aacgccatca ttgagcagtt tatcgactac ctgcgcaccg ggcaggagat
     1981 gccgatggaa atggcggatc ttaatgcagt actcggtgag gtgattgctg ccgaaagtgg
     2041 ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc attgaagtga aaatgcaccc
     2101 gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac gccgcccgtt atggcaatgg
     2161 ctgggtcaaa gtcagcagcg gaacggagcc gaatcgcgcc tggttccagg tggaagatga
     2221 cggtccggga attgcgccgg aacaacgtaa gcacctgttc cagccgtttg tccgcggcga
     2281 cagtgcgcgc accattagcg gcacgggatt agggctggca attgtgcagc gtatcgtgga
     2341 taaccataac gggatgctgg agcttggcac cagcgagcgg ggcgggcttt ccattcgcgc
     2401 ctggctgcca gtgccggtaa cgcgggcgca gggcatgaca aaagaagggt aataactctg
     2461 atagtgctag tgtagatctc
//