##gff-version 3
##sequence-region BBa_S03443 1 1618
BBa_S03443	.	engineered_region	1	1564	.	+	0	ID=BBa_S03442;Name=BBa_S03442
BBa_S03443	.	CDS	22	1564	.	+	0	ID=BBa_C0083;Name=BBa_C0083;Parent=BBa_S03442
BBa_S03443	.	engineered_tag	1540	1564	.	+	0	ID=annotation2213998;Name=Help:Barcodes;Parent=BBa_C0083
BBa_S03443	.	point_mutation	22	22	.	+	0	ID=annotation300982;Name=G;Parent=BBa_C0083
BBa_S03443	.	point_mutation	1020	1020	.	+	0	ID=annotation300987;Name=G;Parent=BBa_C0083
BBa_S03443	.	CDS	22	1500	.	+	0	ID=annotation300981;Name=AspA;Parent=BBa_C0083
BBa_S03443	.	point_mutation	609	609	.	+	0	ID=annotation300983;Name=C;Parent=BBa_C0083
BBa_S03443	.	point_mutation	645	645	.	+	0	ID=annotation300984;Name=G;Parent=BBa_C0083
BBa_S03443	.	point_mutation	1146	1146	.	+	0	ID=annotation300988;Name=G;Parent=BBa_C0083
BBa_S03443	.	point_mutation	678	678	.	+	0	ID=annotation300986;Name=C;Parent=BBa_C0083
BBa_S03443	.	tag	1501	1533	.	+	0	ID=annotation300993;Name=LVA;Parent=BBa_C0083
BBa_S03443	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_S03442
BBa_S03443	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03443	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03443	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03443	.	terminator	1573	1618	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_S03443	.	engineered_region	1573	1618	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_S03443	.	stem_loop	1585	1607	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_S03443
attaaagaggagaaatactagatgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaaca
ttcgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgat
tgaaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagcc
gcagctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcc
tgaacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacac
caacgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgac
catgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagc
tggtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcg
tacccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtg
aaaaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgc
cgaaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgat
cgaagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatc
tgtaacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggct
cttccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacga
caccactgttaccatggcagcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttc
gaatccgttcacattctgaccaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaag
tgtgcgaaggttacgtttacaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacat
cgtgggtaaaatctgtgccgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaa
cttgacgatattttctccgtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacagg
ctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagagagaata
taaaaagccagattattaatccggcttttttattattt