LOCUS       BBa_S03448              1333 bp    DNA     linear       29-Dec-2025
DEFINITION  --Specify Parts List--
ACCESSION   BBa_S03448
VERSION     BBa_S03448.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             378..1196
                     /label=kanamycin resistance
     misc_feature    230..1196
                     /label=BBa_P1003
     stem_loop       1216..1259
                     /label=stem_loop
     misc_feature    1205..1284
                     /label=BBa_B0010
     terminator      1205..1284
                     /label=BBa_B0010
     stem_loop       1300..1319
                     /label=T7 TE
     misc_feature    1326..1326
                     /label=stop
     polyA_site      1320..1333
                     /label=polya
     misc_feature    1293..1333
                     /label=BBa_B0012
     terminator      1293..1333
                     /label=BBa_B0012
     terminator      1205..1333
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagagc tgatccttca
      241 actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt
      301 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
      361 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcc cgtccgcgct
      421 taaactccaa catggacgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
      481 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
      541 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtccgt ctcaactggc
      601 tgacggagtt tatgcctctc ccgaccatca agcattttat ccgtactcct gatgatgcgt
      661 ggttactcac caccgcgatt cctgggaaaa cagccttcca ggtattagaa gaatatcctg
      721 attcaggtga aaatattgtt gatgcgctgg ccgtgttcct gcgccggtta cattcgattc
      781 ctgtttgtaa ttgtcctttt aacagcgatc gtgtatttcg tcttgctcag gcgcaatcac
      841 gcatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
      901 ttgaacaagt ctggaaagaa atgcacaagc tcttgccatt ctcaccggat tcagtcgtca
      961 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     1021 ttgatgttgg acgggtcgga atcgcagacc gttaccagga ccttgccatt ctttggaact
     1081 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     1141 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taataatact
     1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1321 ttctgcgttt ata
//