##gff-version 3
##sequence-region BBa_S03457 1 1747
BBa_S03457	.	engineered_region	846	1747	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_S03457	.	terminator	1645	1685	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_S03457	.	stop_codon	1678	1678	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03457	.	polyA_site	1672	1685	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03457	.	stem_loop	1652	1671	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03457	.	engineered_region	1645	1685	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03457	.	ribosome_entry_site	846	857	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_S03457	.	conserved_region	850	853	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03457	.	promoter	1694	1747	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_S03457	.	engineered_region	1694	1747	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03457	.	promoter	1713	1718	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03457	.	promoter	1736	1741	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03457	.	non_covalent_binding_site	1694	1712	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03457	.	non_covalent_binding_site	1719	1737	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03457	.	CDS	864	1523	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_S03457	.	engineered_tag	1524	1548	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S03457	.	CDS	867	1483	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S03457	.	tag	1484	1517	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S03457	.	terminator	1557	1636	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_S03457	.	engineered_region	1557	1636	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03457	.	stem_loop	1568	1611	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03457	.	engineered_region	124	837	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_S03457	.	sequence_alteration	475	475	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_S03457	.	CDS	124	834	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_S03457	.	promoter	1	117	.	+	0	ID=BBa_J13023;Name=BBa_J13023
BBa_S03457	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_J13023
BBa_S03457	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S03457	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S03457	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S03457	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S03457	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S03457	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S03457	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S03457	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S03457	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13023
BBa_S03457	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03457
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccat
catcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggc
gagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggaca
tcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtc
cttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctcc
ttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaaga
agaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggct
gaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggc
gcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccg
agggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagaaagaggagaaatactagatgtcc
agattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgta
aactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcctt
agccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaat
aacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaa
aacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcact
cagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaaca
cctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttct
tattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaa
ctacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagat
actgagcac