BBa_S03457
type: ComponentDefinition
persistent identity: BBa S03457
display id: BBa_S03457
version: 1
was generated by: iGEM to SBOL conversion
description: --Specify Parts List--
created: 2006-04-13T11:00:00Z
modified: 2015-05-08T01:14:23Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _89_
m user id: 0
owner id: 645
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tami Shinkawa
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q04400
component: BBa_J06504
component: BBa_J13023
sequence annotation: BBa_J13023
sequence annotation: BBa_J06504
sequence annotation: BBa_Q04400
sequence: https://synbiohub.org/public/igem/BBa_S03457_sequence/1

BBa_Q04400
type: Component
persistent identity: component2269462
display id: component2269462
version: 1
top level: BBa_S03457
definition: tetR
access: public

BBa_J06504
type: Component
persistent identity: component2269444
display id: component2269444
version: 1
top level: BBa_S03457
definition: mCherry
access: public

BBa_J13023
type: Component
persistent identity: component2269441
display id: component2269441
version: 1
top level: BBa_S03457
definition: BBa_J13023
access: public

BBa_J13023
type: SequenceAnnotation
persistent identity: annotation2269441
display id: annotation2269441
version: 1
top level: BBa_S03457
location: https://synbiohub.org/public/igem/BBa_S03457/annotation2269441/range2269441/1
component: BBa_J13023

https://synbiohub.org/public/igem/BBa_S03457/annotation2269441/range2269441/1
type: Range
persistent identity: range2269441
display id: range2269441
version: 1
top level: BBa_S03457
direction: direction/reverse
start: 1
end: 117
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation2269444
display id: annotation2269444
version: 1
top level: BBa_S03457
location: https://synbiohub.org/public/igem/BBa_S03457/annotation2269444/range2269444/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_S03457/annotation2269444/range2269444/1
type: Range
persistent identity: range2269444
display id: range2269444
version: 1
top level: BBa_S03457
direction: direction/reverse
start: 124
end: 837
orientation: inline

BBa_Q04400
type: SequenceAnnotation
persistent identity: annotation2269462
display id: annotation2269462
version: 1
top level: BBa_S03457
location: https://synbiohub.org/public/igem/BBa_S03457/annotation2269462/range2269462/1
component: BBa_Q04400

https://synbiohub.org/public/igem/BBa_S03457/annotation2269462/range2269462/1
type: Range
persistent identity: range2269462
display id: range2269462
version: 1
top level: BBa_S03457
direction: direction/reverse
start: 846
end: 1747
orientation: inline

BBa_J13023
type: ComponentDefinition
persistent identity: BBa J13023
display id: BBa_J13023
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC6HSL+LuxR dependent POPS/RIPS generator
created: 2005-06-28T11:00:00Z
modified: 2015-08-31T04:08:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: produces pops and rips in the presence of luxR/3OC6HSL and the absence of lambda cI.
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Jeff Tabor
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0065
component: BBa_B0034
sequence annotation: BBa_R0065
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13023_sequence/1

BBa_R0065
type: Component
persistent identity: component1556571
display id: component1556571
version: 1
top level: BBa_J13023
definition: cI+luxR
access: public

BBa_B0034
type: Component
persistent identity: component1556583
display id: component1556583
version: 1
top level: BBa_J13023
definition: BBa_B0034
access: public

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1556571
display id: annotation1556571
version: 1
top level: BBa_J13023
location: https://synbiohub.org/public/igem/BBa_J13023/annotation1556571/range1556571/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_J13023/annotation1556571/range1556571/1
type: Range
persistent identity: range1556571
display id: range1556571
version: 1
top level: BBa_J13023
direction: direction/forward
start: 1
end: 97
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1556583
display id: annotation1556583
version: 1
top level: BBa_J13023
location: https://synbiohub.org/public/igem/BBa_J13023/annotation1556583/range1556583/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13023/annotation1556583/range1556583/1
type: Range
persistent identity: range1556583
display id: range1556583
version: 1
top level: BBa_J13023
direction: direction/forward
start: 106
end: 117
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: true
creator: ytwang
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa Q04400
display id: BBa_Q04400
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0040.B0015.R0040)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: TetR QPI with strong RBS
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Inverter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0040
component: BBa_R0040
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_Q04400_sequence/1

BBa_B0034
type: Component
persistent identity: component952781
display id: component952781
version: 1
top level: tetR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component952797
display id: component952797
version: 1
top level: tetR
definition: BBa_B0010
access: public

BBa_C0040
type: Component
persistent identity: component952791
display id: component952791
version: 1
top level: tetR
definition: tetR
access: public

BBa_R0040
type: Component
persistent identity: component952822
display id: component952822
version: 1
top level: tetR
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component952807
display id: component952807
version: 1
top level: tetR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952781
display id: annotation952781
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952781/range952781/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04400/annotation952781/range952781/1
type: Range
persistent identity: range952781
display id: range952781
version: 1
top level: tetR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation952791
display id: annotation952791
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952791/range952791/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q04400/annotation952791/range952791/1
type: Range
persistent identity: range952791
display id: range952791
version: 1
top level: tetR
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952797
display id: annotation952797
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952797/range952797/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04400/annotation952797/range952797/1
type: Range
persistent identity: range952797
display id: range952797
version: 1
top level: tetR
direction: direction/forward
start: 712
end: 791
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952807
display id: annotation952807
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952807/range952807/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04400/annotation952807/range952807/1
type: Range
persistent identity: range952807
display id: range952807
version: 1
top level: tetR
direction: direction/forward
start: 800
end: 840
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation952822
display id: annotation952822
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952822/range952822/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q04400/annotation952822/range952822/1
type: Range
persistent identity: range952822
display id: range952822
version: 1
top level: tetR
direction: direction/forward
start: 849
end: 902
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lambda cI and luxR regulated -- hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0065
sequence annotation: Lux Box
sequence annotation: -10
sequence annotation: OR2 cI
sequence annotation: lux p(R) start
sequence annotation: -35
sequence annotation: OR1 cI
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
top level: cI+luxR
direction: direction/forward
start: 1
end: 97
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
top level: cI+luxR
direction: direction/forward
start: 6
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
top level: cI+luxR
direction: direction/forward
start: 47
end: 52
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
top level: cI+luxR
direction: direction/forward
start: 57
end: 73
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
top level: cI+luxR
direction: direction/forward
start: 58
end: 58
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
top level: cI+luxR
direction: direction/forward
start: 71
end: 76
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
top level: cI+luxR
direction: direction/forward
start: 81
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
top level: cI+luxR
direction: direction/forward
start: 94
end: 97
orientation: inline

https://synbiohub.org/public/igem/BBa_S03457_sequence/1
type: Sequence
persistent identity: BBa S03457 sequence
display id: BBa_S03457_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J13023_sequence/1
type: Sequence
persistent identity: BBa J13023 sequence
display id: BBa_J13023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06504_sequence/1
type: Sequence
persistent identity: BBa J06504 sequence
display id: BBa_J06504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04400_sequence/1
type: Sequence
persistent identity: BBa Q04400 sequence
display id: BBa_Q04400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0065_sequence/1
type: Sequence
persistent identity: BBa R0065 sequence
display id: BBa_R0065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z