LOCUS       BBa_S03465              1007 bp    DNA     linear       22-Dec-2024
DEFINITION  Prm+ with CFP as a reporter
ACCESSION   BBa_S03465
VERSION     BBa_S03465.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             109..870
                     /label=CFP (LVA)
     misc_feature    827..864
                     /label=SsrA
     misc_feature    865..870
                     /label=2
     misc_feature    177..177
                     /label=A
     misc_feature    109..870
                     /label=BBa_E0022
     misc_feature    109..870
                     /label=BBa_E0022
     stem_loop       890..933
                     /label=stem_loop
     misc_feature    879..958
                     /label=BBa_B0010
     terminator      879..958
                     /label=BBa_B0010
     stem_loop       974..993
                     /label=T7 TE
     misc_feature    1000..1000
                     /label=stop
     polyA_site      994..1007
                     /label=polya
     misc_feature    967..1007
                     /label=BBa_B0012
     terminator      967..1007
                     /label=BBa_B0012
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag aaagaggaga aatactagat ggtgagcaag
      121 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac
      181 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      241 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      301 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      361 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      421 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      481 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      541 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      601 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      721 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      781 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac
      841 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa
      901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      961 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//