LOCUS       BBa_S03467              1989 bp    DNA     linear       23-Dec-2024
DEFINITION  Pr with CFP and Prm+ with YFP
ACCESSION   BBa_S03467
VERSION     BBa_S03467.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..837
                     /label=CFP (LVA)
     misc_feature    794..831
                     /label=SsrA
     misc_feature    832..837
                     /label=2
     misc_feature    144..144
                     /label=A
     misc_feature    76..837
                     /label=BBa_E0022
     misc_feature    76..837
                     /label=BBa_E0022
     stem_loop       857..900
                     /label=stem_loop
     misc_feature    846..925
                     /label=BBa_B0010
     terminator      846..925
                     /label=BBa_B0010
     stem_loop       941..960
                     /label=T7 TE
     misc_feature    967..967
                     /label=stop
     polyA_site      961..974
                     /label=polya
     misc_feature    934..974
                     /label=BBa_B0012
     terminator      934..974
                     /label=BBa_B0012
     misc_feature    991..1007
                     /label=OR1
     misc_feature    1015..1031
                     /label=OR2
     promoter        1030..1035
                     /label=-35
     misc_feature    1041..1047
                     /label=mutate to obliterate OR3
     promoter        1053..1058
                     /label=-10
     promoter        983..1064
                     /label=BBa_I12007
     misc_feature    1077..1080
                     /label=conserved
     RBS             1073..1084
                     /label=BBa_B0034
     CDS             1091..1852
                     /label=YFP (LVA)
     misc_feature    1847..1852
                     /label=2
     misc_feature    1808..1846
                     /label=SsrA
     misc_feature    1332..1332
                     /label=A (Q->L)
     misc_feature    1091..1852
                     /label=BBa_E0032
     misc_feature    1091..1852
                     /label=BBa_E0032
     stem_loop       1872..1915
                     /label=stem_loop
     misc_feature    1861..1940
                     /label=BBa_B0010
     terminator      1861..1940
                     /label=BBa_B0010
     stem_loop       1956..1975
                     /label=T7 TE
     misc_feature    1982..1982
                     /label=stop
     polyA_site      1976..1989
                     /label=polya
     misc_feature    1949..1989
                     /label=BBa_B0012
     terminator      1949..1989
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac
      301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc
      541 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac
      841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      961 tttctgcgtt tatatactag aggcaaccat tatcaccgcc agaggtaaaa tagtcaacac
     1021 gcacggtgtt agatatttat aaatagtggt gatagattta acgttactag agaaagagga
     1081 gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1141 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1201 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1261 ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc gctaccccga
     1321 ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1381 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1441 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1501 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     1561 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     1621 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1681 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     1741 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1801 gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag
     1861 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1921 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1981 gcgtttata
//