LOCUS BBa_S03470 2373 bp DNA linear 31-Oct-2024 DEFINITION Sensing Device with Pr and GFP ACCESSION BBa_S03470 VERSION BBa_S03470.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 1..1433 /label=BBa_S03334 promoter 1479..1484 /label=-10 promoter 1456..1461 /label=-35 misc_feature 1466..1482 /label=OR1 misc_feature 1442..1458 /label=OR2 misc_feature 1442..1490 /label=BBa_R0051 promoter 1442..1490 /label=BBa_R0051 misc_feature 1503..1506 /label=conserved RBS 1499..1510 /label=BBa_B0034 CDS 1517..2236 /label=GFP protein CDS 1517..2236 /label=BBa_E0040 stem_loop 2256..2299 /label=stem_loop misc_feature 2245..2324 /label=BBa_B0010 terminator 2245..2324 /label=BBa_B0010 stem_loop 2340..2359 /label=T7 TE misc_feature 2366..2366 /label=stop polyA_site 2360..2373 /label=polya misc_feature 2333..2373 /label=BBa_B0012 terminator 2333..2373 /label=BBa_B0012 misc_feature 1442..2373 /label=BBa_S03335 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gtaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc tactagagaa 1501 agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct 1561 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg 1621 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt 1681 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc 1741 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga 1801 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga 1861 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa 1921 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga 1981 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag 2041 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt 2101 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag 2161 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga 2221 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 2281 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 2341 gctcaccttc gggtgggcct ttctgcgttt ata //