LOCUS       BBa_S03471              2415 bp    DNA     linear       31-May-2024
DEFINITION  Sensing Device with Pr and CFP
ACCESSION   BBa_S03471
VERSION     BBa_S03471.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     misc_feature    1381..1397
                     /label=lac O1
     promoter        1398..1403
                     /label=-35
     misc_feature    1404..1420
                     /label=lac O1
     promoter        1421..1426
                     /label=-10
     misc_feature    1379..1432
                     /label=BBa_R0011
     promoter        1379..1432
                     /label=BBa_R0011
     misc_feature    1..1433
                     /label=BBa_S03334
     promoter        1479..1484
                     /label=-10
     promoter        1456..1461
                     /label=-35
     misc_feature    1466..1482
                     /label=OR1
     misc_feature    1442..1458
                     /label=OR2
     misc_feature    1442..1490
                     /label=BBa_R0051
     promoter        1442..1490
                     /label=BBa_R0051
     misc_feature    1503..1506
                     /label=conserved
     RBS             1499..1510
                     /label=BBa_B0034
     CDS             1517..2278
                     /label=CFP (LVA)
     misc_feature    2235..2272
                     /label=SsrA
     misc_feature    2273..2278
                     /label=2
     misc_feature    1585..1585
                     /label=A
     misc_feature    1517..2278
                     /label=BBa_E0022
     misc_feature    1517..2278
                     /label=BBa_E0022
     stem_loop       2298..2341
                     /label=stem_loop
     misc_feature    2287..2366
                     /label=BBa_B0010
     terminator      2287..2366
                     /label=BBa_B0010
     stem_loop       2382..2401
                     /label=T7 TE
     misc_feature    2408..2408
                     /label=stop
     polyA_site      2402..2415
                     /label=polya
     misc_feature    2375..2415
                     /label=BBa_B0012
     terminator      2375..2415
                     /label=BBa_B0012
     misc_feature    1442..2415
                     /label=BBa_S03464
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1441 gtaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc tactagagaa
     1501 agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1561 cctggtcgag ctggacggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga
     1621 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1681 cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta
     1741 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1801 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1861 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1921 caacatcctg gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc
     1981 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
     2041 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2101 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     2161 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2221 cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata
     2281 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2341 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2401 ctttctgcgt ttata
//