##gff-version 3
##sequence-region BBa_S03471 1 2415
BBa_S03471	.	engineered_region	1442	2415	.	+	0	ID=BBa_S03464;Name=BBa_S03464
BBa_S03471	.	terminator	2287	2366	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03464
BBa_S03471	.	engineered_region	2287	2366	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03471	.	stem_loop	2298	2341	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03471	.	terminator	2375	2415	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03464
BBa_S03471	.	engineered_region	2375	2415	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03471	.	stem_loop	2382	2401	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03471	.	stop_codon	2408	2408	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03471	.	polyA_site	2402	2415	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03471	.	promoter	1442	1490	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_S03464
BBa_S03471	.	non_covalent_binding_site	1466	1482	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S03471	.	engineered_region	1442	1490	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S03471	.	promoter	1479	1484	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S03471	.	promoter	1456	1461	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S03471	.	non_covalent_binding_site	1442	1458	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S03471	.	engineered_region	1517	2278	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_S03464
BBa_S03471	.	CDS	1517	2278	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S03471	.	tag	2235	2272	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S03471	.	engineered_region	1517	2278	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S03471	.	point_mutation	1585	1585	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S03471	.	stop_codon	2273	2278	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S03471	.	ribosome_entry_site	1499	1510	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03464
BBa_S03471	.	conserved_region	1503	1506	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03471	.	engineered_region	1	1433	.	+	0	ID=BBa_S03334;Name=BBa_S03334
BBa_S03471	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S03334
BBa_S03471	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S03471	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S03471	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S03471	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S03471	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03334
BBa_S03471	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03471	.	promoter	1379	1432	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_S03334
BBa_S03471	.	operator	1404	1420	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S03471	.	operator	1381	1397	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S03471	.	promoter	1421	1426	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S03471	.	engineered_region	1379	1432	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S03471	.	promoter	1398	1403	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S03471	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03334
BBa_S03471	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03471	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03471	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03471	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03471	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03471	.	terminator	1242	1321	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03334
BBa_S03471	.	engineered_region	1242	1321	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03471	.	stem_loop	1253	1296	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03471	.	terminator	1330	1370	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03334
BBa_S03471	.	engineered_region	1330	1370	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03471	.	stem_loop	1337	1356	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03471	.	stop_codon	1363	1363	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03471	.	polyA_site	1357	1370	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_S03471
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaaga
tactgagcacatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaa
gaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcg
acgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcat
ctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgc
taccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttct
tcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaa
gggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatc
accgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcg
ccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtc
cgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctc
ggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata