LOCUS       BBa_S03472              2392 bp    DNA     linear       22-Dec-2024
DEFINITION  Sensing Device with Prm and RFP
ACCESSION   BBa_S03472
VERSION     BBa_S03472.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     misc_feature    1381..1397
                     /label=lac O1
     promoter        1398..1403
                     /label=-35
     misc_feature    1404..1420
                     /label=lac O1
     promoter        1421..1426
                     /label=-10
     misc_feature    1379..1432
                     /label=BBa_R0011
     promoter        1379..1432
                     /label=BBa_R0011
     misc_feature    1..1433
                     /label=BBa_S03334
     misc_feature    1450..1466
                     /label=OR1
     misc_feature    1474..1490
                     /label=OR2
     promoter        1489..1494
                     /label=-35
     misc_feature    1500..1506
                     /label=mutate to obliterate OR3
     promoter        1512..1517
                     /label=-10
     promoter        1442..1523
                     /label=BBa_I12007
     misc_feature    1536..1539
                     /label=conserved
     RBS             1532..1543
                     /label=BBa_B0034
     CDS             1550..2224
                     /label=mrfp1
     misc_feature    2231..2255
                     /label=Help:Barcodes
     CDS             1550..2230
                     /label=BBa_E1010
     stem_loop       2275..2318
                     /label=stem_loop
     misc_feature    2264..2343
                     /label=BBa_B0010
     terminator      2264..2343
                     /label=BBa_B0010
     stem_loop       2359..2378
                     /label=T7 TE
     misc_feature    2385..2385
                     /label=stop
     polyA_site      2379..2392
                     /label=polya
     misc_feature    2352..2392
                     /label=BBa_B0012
     terminator      2352..2392
                     /label=BBa_B0012
     misc_feature    1442..2392
                     /label=BBa_S03337
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1441 ggcaaccatt atcaccgcca gaggtaaaat agtcaacacg cacggtgtta gatatttata
     1501 aatagtggtg atagatttaa cgttactaga gaaagaggag aaatactaga tggcttcctc
     1561 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
     1621 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
     1681 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
     1741 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
     1801 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
     1861 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
     1921 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
     1981 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
     2041 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
     2101 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     2161 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     2221 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
     2281 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2341 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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