SD+Prm+RFP
type: ComponentDefinition
persistent identity: BBa S03472
display id: BBa_S03472
version: 1
was generated by: iGEM to SBOL conversion
description: Sensing Device with Prm and RFP
created: 2006-05-01T11:00:00Z
modified: 2015-05-08T01:14:23Z
mutable provenance: Registry 7.05
owned by: james
owned by: myers
mutable description: To test the controllability/leakiness of the lambda-system and reporter detectability.
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.
star: false
creator: ETH Zurich iGEM Team 2005
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_S03334
component: BBa_S03337
sequence annotation: BBa_S03334
sequence annotation: BBa_S03337
sequence: https://synbiohub.org/public/igem/BBa_S03472_sequence/1

BBa_S03334
type: Component
persistent identity: component2291170
display id: component2291170
version: 1
top level: SD+Prm+RFP
definition: input-lacI
access: public

BBa_S03337
type: Component
persistent identity: component2291188
display id: component2291188
version: 1
top level: SD+Prm+RFP
definition: Prm+RFP
access: public

BBa_S03334
type: SequenceAnnotation
persistent identity: annotation2291170
display id: annotation2291170
version: 1
top level: SD+Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03472/annotation2291170/range2291170/1
component: BBa_S03334

https://synbiohub.org/public/igem/BBa_S03472/annotation2291170/range2291170/1
type: Range
persistent identity: range2291170
display id: range2291170
version: 1
top level: SD+Prm+RFP
direction: direction/reverse
start: 1
end: 1433
orientation: inline

BBa_S03337
type: SequenceAnnotation
persistent identity: annotation2291188
display id: annotation2291188
version: 1
top level: SD+Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03472/annotation2291188/range2291188/1
component: BBa_S03337

https://synbiohub.org/public/igem/BBa_S03472/annotation2291188/range2291188/1
type: Range
persistent identity: range2291188
display id: range2291188
version: 1
top level: SD+Prm+RFP
direction: direction/reverse
start: 1442
end: 2392
orientation: inline

input-lacI
type: ComponentDefinition
persistent identity: BBa S03334
display id: BBa_S03334
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive lacI-production to block cI production.
created: 2005-09-20T11:00:00Z
modified: 2015-05-08T01:14:21Z
mutable provenance: Registry 7.05
owned by: james
owned by: myers
mutable description: Constitutive (tetR) lacI-production (lacI+LVA) to block promoter (lacI+pL) in ground state of a 2-state system. lacI+pL can be activated, e.g. over IPTG, thus leading to the production of whatever comes after it (planned: cI production as regulator in INPUT-module).
discontinued: false
dominant: false
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
star: false
creator: ETH Zurich iGEM Team 2005
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0040
component: BBa_C0012
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0011
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_S03334_sequence/1

BBa_R0040
type: Component
persistent identity: component1700035
display id: component1700035
version: 1
top level: input-lacI
definition: p(tetR)
access: public

BBa_C0012
type: Component
persistent identity: component1700053
display id: component1700053
version: 1
top level: input-lacI
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component1700043
display id: component1700043
version: 1
top level: input-lacI
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1700070
display id: component1700070
version: 1
top level: input-lacI
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1700060
display id: component1700060
version: 1
top level: input-lacI
definition: BBa_B0010
access: public

BBa_R0011
type: Component
persistent identity: component1700084
display id: component1700084
version: 1
top level: input-lacI
definition: lacI+pL
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1700035
display id: annotation1700035
version: 1
top level: input-lacI
location: https://synbiohub.org/public/igem/BBa_S03334/annotation1700035/range1700035/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03334/annotation1700035/range1700035/1
type: Range
persistent identity: range1700035
display id: range1700035
version: 1
top level: input-lacI
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1700043
display id: annotation1700043
version: 1
top level: input-lacI
location: https://synbiohub.org/public/igem/BBa_S03334/annotation1700043/range1700043/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03334/annotation1700043/range1700043/1
type: Range
persistent identity: range1700043
display id: range1700043
version: 1
top level: input-lacI
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1700053
display id: annotation1700053
version: 1
top level: input-lacI
location: https://synbiohub.org/public/igem/BBa_S03334/annotation1700053/range1700053/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_S03334/annotation1700053/range1700053/1
type: Range
persistent identity: range1700053
display id: range1700053
version: 1
top level: input-lacI
direction: direction/forward
start: 81
end: 1208
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1700060
display id: annotation1700060
version: 1
top level: input-lacI
location: https://synbiohub.org/public/igem/BBa_S03334/annotation1700060/range1700060/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03334/annotation1700060/range1700060/1
type: Range
persistent identity: range1700060
display id: range1700060
version: 1
top level: input-lacI
direction: direction/forward
start: 1242
end: 1321
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1700070
display id: annotation1700070
version: 1
top level: input-lacI
location: https://synbiohub.org/public/igem/BBa_S03334/annotation1700070/range1700070/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03334/annotation1700070/range1700070/1
type: Range
persistent identity: range1700070
display id: range1700070
version: 1
top level: input-lacI
direction: direction/forward
start: 1330
end: 1370
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1700084
display id: annotation1700084
version: 1
top level: input-lacI
location: https://synbiohub.org/public/igem/BBa_S03334/annotation1700084/range1700084/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_S03334/annotation1700084/range1700084/1
type: Range
persistent identity: range1700084
display id: range1700084
version: 1
top level: input-lacI
direction: direction/forward
start: 1379
end: 1432
orientation: inline

Prm+RFP
type: ComponentDefinition
persistent identity: BBa S03337
display id: BBa_S03337
version: 1
was generated by: iGEM to SBOL conversion
description: Prm+ with RFP as a reporter
created: 2005-09-20T11:00:00Z
modified: 2015-05-08T01:14:21Z
mutable provenance: Registry 7.05
owned by: james
owned by: myers
mutable description: Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with RFP as a reporter. It is needed in subsequent parts to observe the performance of the complete input-system, which is based on the modified lambda-system.
discontinued: false
dominant: false
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
star: false
creator: ETH Zurich iGEM Team 2005
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
component: BBa_B0034
component: BBa_I12007
sequence annotation: BBa_I12007
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03337_sequence/1

BBa_B0010
type: Component
persistent identity: component1700414
display id: component1700414
version: 1
top level: Prm+RFP
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1700408
display id: component1700408
version: 1
top level: Prm+RFP
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1700424
display id: component1700424
version: 1
top level: Prm+RFP
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1700401
display id: component1700401
version: 1
top level: Prm+RFP
definition: BBa_B0034
access: public

BBa_I12007
type: Component
persistent identity: component1700396
display id: component1700396
version: 1
top level: Prm+RFP
definition: Prm +
access: public

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1700396
display id: annotation1700396
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700396/range1700396/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_S03337/annotation1700396/range1700396/1
type: Range
persistent identity: range1700396
display id: range1700396
version: 1
top level: Prm+RFP
direction: direction/forward
start: 1
end: 82
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1700401
display id: annotation1700401
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700401/range1700401/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03337/annotation1700401/range1700401/1
type: Range
persistent identity: range1700401
display id: range1700401
version: 1
top level: Prm+RFP
direction: direction/forward
start: 91
end: 102
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1700408
display id: annotation1700408
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700408/range1700408/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_S03337/annotation1700408/range1700408/1
type: Range
persistent identity: range1700408
display id: range1700408
version: 1
top level: Prm+RFP
direction: direction/forward
start: 109
end: 789
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1700414
display id: annotation1700414
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700414/range1700414/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03337/annotation1700414/range1700414/1
type: Range
persistent identity: range1700414
display id: range1700414
version: 1
top level: Prm+RFP
direction: direction/forward
start: 823
end: 902
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1700424
display id: annotation1700424
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700424/range1700424/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03337/annotation1700424/range1700424/1
type: Range
persistent identity: range1700424
display id: range1700424
version: 1
top level: Prm+RFP
direction: direction/forward
start: 911
end: 951
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lambda Prm promoter (OR-3 obliterated)
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
star: true
creator: Hans
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: -35
sequence annotation: mutate to obliterate OR3
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
top level: Prm +
direction: direction/forward
start: 59
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

https://synbiohub.org/public/igem/BBa_S03472_sequence/1
type: Sequence
persistent identity: BBa S03472 sequence
display id: BBa_S03472_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03334_sequence/1
type: Sequence
persistent identity: BBa S03334 sequence
display id: BBa_S03334_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03337_sequence/1
type: Sequence
persistent identity: BBa S03337 sequence
display id: BBa_S03337_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12007_sequence/1
type: Sequence
persistent identity: BBa I12007 sequence
display id: BBa_I12007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z