##gff-version 3
##sequence-region BBa_S03491 1 855
BBa_S03491	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_S03491	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_S03491	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_S03491	.	engineered_region	58	855	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_S03491	.	CDS	76	693	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_S03491	.	tag	655	686	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S03491	.	engineered_region	76	693	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S03491	.	engineered_tag	694	718	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S03491	.	CDS	76	654	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S03491	.	terminator	727	806	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_S03491	.	engineered_region	727	806	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03491	.	stem_loop	738	781	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03491	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_S03491	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03491	.	terminator	815	855	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_S03491	.	engineered_region	815	855	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03491	.	polyA_site	842	855	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03491	.	stop_codon	848	848	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03491	.	stem_loop	822	841	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_S03491
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatga
ctataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagt
gtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaa
tatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctga
aaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgt
aggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgct
gttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttcctt
gtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaaca
gtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata