##gff-version 3
##sequence-region BBa_S03514 1 749
BBa_S03514	.	CDS	1	612	.	+	0	ID=BBa_J31001;Name=BBa_J31001
BBa_S03514	.	stop_codon	610	612	.	+	0	ID=annotation1910567;Name=Stop codon;Parent=BBa_J31001
BBa_S03514	.	CDS	1	570	.	+	0	ID=annotation1910564;Name=Hin;Parent=BBa_J31001
BBa_S03514	.	tag	571	609	.	+	0	ID=annotation1910566;Name=LVA;Parent=BBa_J31001
BBa_S03514	.	primer_binding_site	1	3	.	+	0	ID=annotation1910565;Name=Fwd primer J31001;Parent=BBa_J31001
BBa_S03514	.	primer_binding_site	551	612	.	+	0	ID=annotation1910570;Name=Rev primer J31001;Parent=BBa_J31001
BBa_S03514	.	terminator	621	749	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S03514	.	terminator	621	700	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S03514	.	stem_loop	632	675	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03514	.	engineered_region	621	700	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03514	.	terminator	709	749	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S03514	.	stem_loop	716	735	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03514	.	stop_codon	742	742	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03514	.	polyA_site	736	749	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03514	.	engineered_region	709	749	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S03514
atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaa
attgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgt
aaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatca
gaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattctttt
ttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccag
agcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaa
ggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgta
taaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata