LOCUS       BBa_S03515               710 bp    DNA     linear       16-Feb-2025
DEFINITION  Hin : TT
ACCESSION   BBa_S03515
VERSION     BBa_S03515.1  
FEATURES             Location/Qualifiers
     misc_feature    70..75
                     /label=Former SpeI site
     misc_feature    72..72
                     /label=Mutation to delete the SpeI site
     CDS             1..573
                     /label=Hin sequence
     primer_bind     1..3
                     /label=Fwd primer J31001
     primer_bind     551..570
                     /label=Rev primer J31001
     CDS             1..573
                     /label=BBa_J31000
     stem_loop       593..636
                     /label=stem_loop
     misc_feature    582..661
                     /label=BBa_B0010
     terminator      582..661
                     /label=BBa_B0010
     stem_loop       677..696
                     /label=T7 TE
     misc_feature    703..703
                     /label=stop
     polyA_site      697..710
                     /label=polya
     misc_feature    670..710
                     /label=BBa_B0012
     terminator      670..710
                     /label=BBa_B0012
ORIGIN
        1 atggctacta ttgggtatat tcgggtgtca acaattgacc aaaatatcga tttacagcgt
       61 aatgcgctta ccagtgcaaa ttgtgaccgc atttttgaag accgtatcag tggcaagatt
      121 gcaaaccgcc ccggcctgaa acgggcgtta aagtatgtaa ataaaggcga tactcttgtc
      181 gtctggaaat tagacagact gggccgtagc gtgaaaaatc tggtggcgtt aatatcagaa
      241 ttacatgaac gtggagctca cttccattct ttaaccgata gtattgatac cagtagcgcg
      301 atggggcgat tcttttttca tgtaatgtca gcactggccg agatggagcg agaattaatc
      361 gtcgagcgaa cccttgccgg actggctgcc gccagagcgc aaggacgact gggagggcgc
      421 cctcgggcga tcaacaaaca tgaacaggaa cagattagtc ggctattaga gaaaggccat
      481 cctcggcagc aattagctat tatttttggt attggcgtat ccaccttata cagatacttt
      541 ccggcaagca gtataaaaaa acgaatgaat taatactaga gccaggcatc aaataaaacg
      601 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      661 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//