##gff-version 3
##sequence-region BBa_S03515 1 710
BBa_S03515	.	terminator	670	710	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03515	.	polyA_site	697	710	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03515	.	engineered_region	670	710	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03515	.	stem_loop	677	696	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03515	.	stop_codon	703	703	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03515	.	terminator	582	661	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03515	.	stem_loop	593	636	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03515	.	engineered_region	582	661	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03515	.	CDS	1	573	.	+	0	ID=BBa_J31000;Name=BBa_J31000
BBa_S03515	.	CDS	1	573	.	+	0	ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000
BBa_S03515	.	sequence_alteration	70	75	.	+	0	ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000
BBa_S03515	.	primer_binding_site	551	570	.	+	0	ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000
BBa_S03515	.	primer_binding_site	1	3	.	+	0	ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000
BBa_S03515	.	sequence_alteration	72	72	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000
>BBa_S03515
atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaa
attgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgt
aaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatca
gaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattctttt
ttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccag
agcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaa
ggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagta
taaaaaaacgaatgaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata