##gff-version 3
##sequence-region BBa_S03524 1 1447
BBa_S03524	.	terminator	1322	1362	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03524	.	stop_codon	1355	1355	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03524	.	engineered_region	1322	1362	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03524	.	stem_loop	1329	1348	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03524	.	polyA_site	1349	1362	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03524	.	CDS	1	1191	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S03524	.	CDS	1	1191	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03524	.	primer_binding_site	1172	1191	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03524	.	primer_binding_site	1	24	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03524	.	stop_codon	1189	1191	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03524	.	sequence_feature	1371	1447	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_S03524	.	non_covalent_binding_site	1425	1439	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03524	.	sequence_feature	1371	1447	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03524	.	sequence_feature	1421	1426	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03524	.	sequence_feature	1399	1399	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03524	.	sequence_feature	1447	1447	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03524	.	non_covalent_binding_site	1376	1391	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03524	.	sequence_feature	1421	1421	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03524	.	sequence_feature	1200	1225	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03524	.	terminator	1234	1313	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03524	.	stem_loop	1245	1288	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03524	.	engineered_region	1234	1313	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S03524
atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccgg
tactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgc
gttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcg
ctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtgg
ccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgcca
cttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctcc
ttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgc
ataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactat
cgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggc
gaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaag
ccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctggg
ctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcggg
atgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctc
ttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggtt
ggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcg
acctaatactagagttatcaaaaaccatggtttttgataatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttc
tagtgcaaattgtgaccgcattttg