##gff-version 3
##sequence-region BBa_S03526 1 1447
BBa_S03526	.	engineered_region	1234	1447	.	+	0	ID=BBa_S03516;Name=BBa_S03516
BBa_S03526	.	sequence_feature	1371	1447	.	+	0	ID=BBa_J3101;Name=BBa_J3101;Parent=BBa_S03516
BBa_S03526	.	sequence_feature	1399	1399	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03526	.	sequence_feature	1371	1447	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03526	.	sequence_feature	1421	1426	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03526	.	sequence_feature	1421	1421	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03526	.	non_covalent_binding_site	1425	1439	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03526	.	sequence_feature	1447	1447	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03526	.	non_covalent_binding_site	1376	1391	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03526	.	terminator	1234	1313	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03516
BBa_S03526	.	engineered_region	1234	1313	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03526	.	stem_loop	1245	1288	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03526	.	terminator	1322	1362	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03516
BBa_S03526	.	engineered_region	1322	1362	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03526	.	stop_codon	1355	1355	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03526	.	stem_loop	1329	1348	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03526	.	polyA_site	1349	1362	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03526	.	engineered_region	1	1225	.	+	0	ID=BBa_S03525;Name=BBa_S03525
BBa_S03526	.	sequence_feature	1200	1225	.	+	0	ID=BBa_J44000;Name=BBa_J44000;Parent=BBa_S03525
BBa_S03526	.	CDS	1	1191	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S03525
BBa_S03526	.	CDS	1	1191	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S03526	.	stop_codon	1	3	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S03526	.	primer_binding_site	1	20	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S03526	.	primer_binding_site	1168	1191	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
>BBa_S03526
ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatc
catgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggc
tggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgc
gggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaag
acgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtga
cgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcg
gtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcg
gcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctc
ccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgc
atgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagc
ccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtga
tgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctcc
aagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgc
atcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccg
gcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttaga
tttcattactagagttatcaaaaaccatggtttttgataatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttc
tagtgcaaattgtgaccgcattttg