##gff-version 3
##sequence-region BBa_S03527 1 1479
BBa_S03527	.	sequence_feature	1	26	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03527	.	CDS	33	1223	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S03527	.	CDS	33	1223	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03527	.	primer_binding_site	1204	1223	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03527	.	primer_binding_site	33	56	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03527	.	stop_codon	1221	1223	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03527	.	sequence_feature	1232	1257	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03527	.	terminator	1266	1345	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03527	.	stem_loop	1277	1320	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03527	.	engineered_region	1266	1345	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03527	.	terminator	1354	1394	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03527	.	stem_loop	1361	1380	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03527	.	stop_codon	1387	1387	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03527	.	engineered_region	1354	1394	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03527	.	polyA_site	1381	1394	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03527	.	sequence_feature	1403	1479	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_S03527	.	sequence_feature	1479	1479	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03527	.	sequence_feature	1403	1479	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03527	.	sequence_feature	1453	1458	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03527	.	sequence_feature	1453	1453	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03527	.	non_covalent_binding_site	1457	1471	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03527	.	sequence_feature	1431	1431	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03527	.	non_covalent_binding_site	1408	1423	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
>BBa_S03527
ttatcaaaaaccatggtttttgataatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccct
ggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgcc
agtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgacc
gctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgt
cctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgcc
gacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggcc
ccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacct
actactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtc
agctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggac
aggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgc
ggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggcc
attatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttcccca
ttatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgacca
tcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatt
tatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgt
tgcgtcgcggtgcatggagccgggccacctcgacctaatactagagttatcaaaaaccatggtttttgataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattgac
caaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg