##gff-version 3
##sequence-region BBa_S03528 1 1481
BBa_S03528	.	engineered_region	35	1481	.	+	0	ID=BBa_S03526;Name=BBa_S03526
BBa_S03528	.	engineered_region	1268	1481	.	+	0	ID=BBa_S03516;Name=BBa_S03516;Parent=BBa_S03526
BBa_S03528	.	sequence_feature	1405	1481	.	+	0	ID=BBa_J3101;Name=BBa_J3101;Parent=BBa_S03516
BBa_S03528	.	sequence_feature	1481	1481	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03528	.	sequence_feature	1455	1460	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03528	.	sequence_feature	1433	1433	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03528	.	sequence_feature	1455	1455	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03528	.	non_covalent_binding_site	1459	1473	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03528	.	sequence_feature	1405	1481	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03528	.	non_covalent_binding_site	1410	1425	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03528	.	terminator	1356	1396	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03516
BBa_S03528	.	stem_loop	1363	1382	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03528	.	polyA_site	1383	1396	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03528	.	stop_codon	1389	1389	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03528	.	engineered_region	1356	1396	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03528	.	terminator	1268	1347	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03516
BBa_S03528	.	stem_loop	1279	1322	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03528	.	engineered_region	1268	1347	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03528	.	engineered_region	35	1259	.	+	0	ID=BBa_S03525;Name=BBa_S03525;Parent=BBa_S03526
BBa_S03528	.	CDS	35	1225	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S03525
BBa_S03528	.	primer_binding_site	35	54	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S03528	.	primer_binding_site	1202	1225	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S03528	.	CDS	35	1225	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S03528	.	stop_codon	35	37	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S03528	.	sequence_feature	1234	1259	.	+	0	ID=BBa_J44000;Name=BBa_J44000;Parent=BBa_S03525
BBa_S03528	.	sequence_feature	1	26	.	+	0	ID=BBa_J44000;Name=BBa_J44000
>BBa_S03528
ttatcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggg
gaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgcc
gtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgt
catctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaa
ggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgc
ttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcg
acaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctac
gagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactggg
ttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttga
ggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgc
caccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgata
taggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtg
tggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcgga
cagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatg
ctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtga
cggtgccgaggatgacgatgagcgcattgttagatttcattactagagttatcaaaaaccatggtttttgataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattg
accaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg