##gff-version 3
##sequence-region BBa_S03534 1 1470
BBa_S03534	.	engineered_region	1223	1470	.	+	0	ID=BBa_S03522;Name=BBa_S03522
BBa_S03534	.	terminator	1345	1385	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03522
BBa_S03534	.	stop_codon	1378	1378	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03534	.	stem_loop	1352	1371	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03534	.	engineered_region	1345	1385	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03534	.	polyA_site	1372	1385	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03534	.	sequence_feature	1223	1248	.	+	0	ID=BBa_J44000;Name=BBa_J44000;Parent=BBa_S03522
BBa_S03534	.	sequence_feature	1394	1470	.	+	0	ID=BBa_J3101;Name=BBa_J3101;Parent=BBa_S03522
BBa_S03534	.	non_covalent_binding_site	1448	1462	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03534	.	sequence_feature	1422	1422	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03534	.	sequence_feature	1470	1470	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03534	.	sequence_feature	1444	1444	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03534	.	sequence_feature	1394	1470	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03534	.	non_covalent_binding_site	1399	1414	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03534	.	sequence_feature	1444	1449	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03534	.	terminator	1257	1336	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03522
BBa_S03534	.	stem_loop	1268	1311	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03534	.	engineered_region	1257	1336	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03534	.	engineered_region	1	1214	.	+	0	ID=BBa_S03532;Name=BBa_S03532
BBa_S03534	.	ribosome_entry_site	1200	1214	.	+	0	ID=BBa_J44001;Name=BBa_J44001;Parent=BBa_S03532
BBa_S03534	.	ribosome_entry_site	1200	1214	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_S03534	.	CDS	1	1191	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S03532
BBa_S03534	.	stop_codon	1	3	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S03534	.	CDS	1	1191	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S03534	.	primer_binding_site	1168	1191	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S03534	.	primer_binding_site	1	20	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
>BBa_S03534
ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatc
catgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggc
tggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgc
gggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaag
acgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtga
cgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcg
gtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcg
gcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctc
ccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgc
atgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagc
ccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtga
tgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctcc
aagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgc
atcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccg
gcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttaga
tttcattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattgaccaaaatatt
gatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg