LOCUS       BBa_S03536               978 bp    DNA     linear       26-Nov-2024
DEFINITION  pLac-RBS-HinLVA-TT
ACCESSION   BBa_S03536
VERSION     BBa_S03536.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    1..223
                     /label=BBa_S03535
     CDS             230..799
                     /label=Hin
     primer_bind     230..232
                     /label=Fwd primer J31001
     misc_feature    800..838
                     /label=LVA
     misc_feature    839..841
                     /label=Stop codon
     primer_bind     780..841
                     /label=Rev primer J31001
     CDS             230..841
                     /label=BBa_J31001
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     terminator      850..978
                     /label=BBa_B0015
     misc_feature    230..978
                     /label=BBa_S03514
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctactat
      241 tgggtatatt cgggtgtcaa caattgacca aaatatcgat ttacagcgta atgcgcttac
      301 cagtgcaaat tgtgaccgca tttttgagga ccgtatcagt ggcaagattg caaaccgccc
      361 cggcctgaaa cgagcgttaa agtatgtaaa taaaggcgat actcttgtcg tctggaaatt
      421 agacagactg ggccgcagcg tgaaaaacct ggtggcgtta atatcagaat tacatgaacg
      481 tggagctcac ttccattctt taaccgatag tattgatacc agtagcgcga tggggcgatt
      541 cttttttcat gtaatgtcag cactggccga gatggagcga gaattaattg tcgagcgaac
      601 ccttgccgga ctggctgccg ccagagcgca aggacgactg ggagggcgcc ctcgggcgat
      661 caacagacat gaacaggaac agattagtcg gctattagag aaaggccatc ctcggcagca
      721 actagctatt atttttggta ttggcgtatc taccttatac agatattttc cggcaagccg
      781 tataaaaaaa cgaatgaata ggcctgctgc aaacgacgaa aactacgctt tagtagctta
      841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttata
//