##gff-version 3
##sequence-region BBa_S03537 1 939
BBa_S03537	.	CDS	230	802	.	+	0	ID=BBa_J31000;Name=BBa_J31000
BBa_S03537	.	CDS	230	802	.	+	0	ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000
BBa_S03537	.	sequence_alteration	299	304	.	+	0	ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000
BBa_S03537	.	primer_binding_site	780	799	.	+	0	ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000
BBa_S03537	.	primer_binding_site	230	232	.	+	0	ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000
BBa_S03537	.	sequence_alteration	301	301	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000
BBa_S03537	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S03537	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03537	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03537	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03537	.	terminator	899	939	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03537	.	polyA_site	926	939	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03537	.	engineered_region	899	939	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03537	.	stem_loop	906	925	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03537	.	stop_codon	932	932	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03537	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S03537	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S03537	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S03537	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S03537	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S03537	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S03537	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S03537	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S03537	.	terminator	811	890	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03537	.	stem_loop	822	865	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03537	.	engineered_region	811	890	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S03537
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctac
tattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgac
cgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaag
gcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattaca
tgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgta
atgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaag
gacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcc
tcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaa
cgaatgaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata