LOCUS       BBa_S03546              1757 bp    DNA     linear       22-Dec-2024
DEFINITION  C0260:E0422
ACCESSION   BBa_S03546
VERSION     BBa_S03546.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=start
     misc_feature    802..807
                     /label=2
     misc_feature    769..801
                     /label=LVA
     CDS             19..768
                     /label=aiiA
     misc_feature    19..807
                     /label=BBa_C0060
     misc_feature    808..832
                     /label=Help:Barcodes
     CDS             19..807
                     /label=BBa_C0060
     misc_feature    1..832
                     /label=BBa_C0260
     misc_feature    845..848
                     /label=conserved
     RBS             841..852
                     /label=BBa_B0034
     CDS             859..1620
                     /label=CFP (LVA)
     misc_feature    1577..1614
                     /label=SsrA
     misc_feature    1615..1620
                     /label=2
     misc_feature    927..927
                     /label=A
     misc_feature    859..1620
                     /label=BBa_E0022
     misc_feature    859..1620
                     /label=BBa_E0022
     stem_loop       1640..1683
                     /label=stem_loop
     misc_feature    1629..1708
                     /label=BBa_B0010
     terminator      1629..1708
                     /label=BBa_B0010
     stem_loop       1724..1743
                     /label=T7 TE
     misc_feature    1750..1750
                     /label=stop
     polyA_site      1744..1757
                     /label=polya
     misc_feature    1717..1757
                     /label=BBa_B0012
     terminator      1717..1757
                     /label=BBa_B0012
     misc_feature    841..1757
                     /label=BBa_E0422
ORIGIN
        1 aaagaggaga aatactagat gacagtaaag aagctttatt tcgtcccagc aggtcgttgt
       61 atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt agacttaccg
      121 gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac aggtatgcca
      181 gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg gcaggtttta
      241 ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg ttatgagccg
      301 gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg aggaaatggc
      361 gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc gcagcatagc
      421 gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat tgaaggtgat
      481 tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc agggcatcaa
      541 tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga tgcatcgtat
      601 acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga attagcttta
      661 tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt tttctttgga
      721 catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc tgcaaacgac
      781 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag
      841 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      901 atcctggtcg agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc
      961 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1021 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc
     1081 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1141 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1201 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1261 ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc
     1321 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     1381 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1441 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1501 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1561 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
     1621 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1681 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1741 gcctttctgc gtttata
//