##gff-version 3
##sequence-region BBa_S03546 1 1757
BBa_S03546	.	engineered_region	841	1757	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_S03546	.	terminator	1629	1708	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_S03546	.	engineered_region	1629	1708	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03546	.	stem_loop	1640	1683	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03546	.	terminator	1717	1757	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_S03546	.	engineered_region	1717	1757	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03546	.	stop_codon	1750	1750	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03546	.	stem_loop	1724	1743	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03546	.	polyA_site	1744	1757	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03546	.	engineered_region	859	1620	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_S03546	.	CDS	859	1620	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S03546	.	tag	1577	1614	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S03546	.	point_mutation	927	927	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S03546	.	stop_codon	1615	1620	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S03546	.	engineered_region	859	1620	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S03546	.	ribosome_entry_site	841	852	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_S03546	.	conserved_region	845	848	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03546	.	engineered_region	1	832	.	+	0	ID=BBa_C0260;Name=BBa_C0260
BBa_S03546	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0260
BBa_S03546	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03546	.	CDS	19	807	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_C0260
BBa_S03546	.	engineered_region	19	807	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S03546	.	start_codon	19	21	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S03546	.	engineered_tag	808	832	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S03546	.	CDS	19	768	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S03546	.	tag	769	801	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S03546	.	stop_codon	802	807	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
>BBa_S03546
aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctg
ttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctat
tttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggtt
ttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttata
ttattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgc
tgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaa
ggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattg
agacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatt
tgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgtt
ttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacg
ctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggtgagcaa
gggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtg
tccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgc
cctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacga
cttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacc
cgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggca
acatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcat
caaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccccc
atcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgaga
agcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcc
tgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttata